Wang Ying, Chen Hanlu, Liu Xiaoli, Song Jingxuan, Xu Senxin, Abulaiti Reziwanguli, Han Dongmei, Hu Yang, Wang Zhihua, Gu Fubo
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Quzhou Institute for Innovation in Resource Chemical Engineering, Quzhou 324000, China.
Carbohydr Polym. 2025 Mar 15;352:123152. doi: 10.1016/j.carbpol.2024.123152. Epub 2024 Dec 18.
Hermetia illucens, with a short growth cycle, is promising as a valuable source of chitin. However, the optimal method for extracting chitin from this insect and its application for hemostasis has not been addressed. This work employed an environmentally friendly choline chloride-lactic acid deep eutectic solvent technology to extract chitin effectively from the Hermetia illucens pupae shells, realizing one-step removal of inorganic salts and proteins. The extraction process was optimized through the response surface methodology, which identified the optimal temperature (92 °C), time (5.6 h), and liquid-solid ratio (20 mL/g) as the conditions that yielded the highest chitin yield (28.40 %) and the highest purity (96.93 %). The structural characterization of the extracted chitin revealed an α-type structure with favorable thermal stability. Furthermore, by regulating the degree of deacetylation, we optimized the degree of protonation of chitin for producing a series of chitosan (Cts), thereby enabling the production of Cts2 with excellent in vitro hemostatic properties. The superior hemostatic performance of Cts2 can be attributed to significantly superior platelet adhesion, achieved preferred adsorption of fibrinogen, and improved blood coagulation. This work provides a theoretical basis for the efficient extraction and high-value application of chitin from Hermetia illucens.
黑水虻生长周期短，有望成为甲壳素的宝贵来源。然而，从这种昆虫中提取甲壳素的最佳方法及其止血应用尚未得到解决。这项工作采用了环境友好的氯化胆碱-乳酸低共熔溶剂技术，从黑水虻蛹壳中有效提取甲壳素，实现了无机盐和蛋白质的一步去除。通过响应面法对提取工艺进行了优化，确定了最佳温度（92℃）、时间（5.6小时）和液固比（20mL/g），这些条件下甲壳素产量最高（28.40%），纯度最高（96.93%）。提取的甲壳素的结构表征显示为具有良好热稳定性的α型结构。此外，通过调节脱乙酰度，我们优化了甲壳素的质子化程度以生产一系列壳聚糖（Cts），从而能够生产出具有优异体外止血性能的Cts2。Cts2优异的止血性能可归因于显著优越的血小板粘附、实现了对纤维蛋白原的优先吸附以及改善了血液凝固。这项工作为从黑水虻中高效提取甲壳素及其高值化应用提供了理论依据。
