Watanabe Masaki, Takimoto Hayato R, Hashimoto Kazuki, Ishii Yuki, Sasaki Nobuya
Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine, Kitasato University, Towada, Japan.
Animal Model Exp Med. 2025 Mar;8(3):568-572. doi: 10.1002/ame2.12553. Epub 2025 Jan 22.
This study aimed to investigate the impact of administration routes in establishing the Adriamycin (ADR)-induced chronic kidney disease (CKD) model. Using BALB/c mice, we compared the effects of conventional tail-vein injection (TV10, 10 mg/kg) to those of retro-orbital sinus (orbital vein) injection (OV10, 10 mg/kg; OV8, 8 mg/kg). The results indicated that the OV10 group exhibited CKD pathology similar to the TV10 group, with both groups demonstrating significantly higher urinary albumin/creatinine ratio (p < 0.05), tubular injury (p < 0.05), and degree of renal fibrosis (p < 0.05) than the OV8 group. No significant differences were observed between the OV10 and TV10 groups in urinary albumin/creatinine ratio, tubular injury, and degree of renal fibrosis. These findings demonstrated that retro-orbital administration of 10 mg/kg ADR induces comparable effects to conventional tail-vein administration. This technique's technical simplicity may improve experimental efficiency, reproducibility, and animal welfare in CKD research. In conclusion, this study validates the utility of retro-orbital injection in CKD model establishment, demonstrating its potential to standardize and improve the reliability of future CKD research protocols.
本研究旨在探讨给药途径对建立阿霉素（ADR）诱导的慢性肾脏病（CKD）模型的影响。我们使用BALB/c小鼠，比较了传统尾静脉注射（TV10，10mg/kg）与眶后窦（眶静脉）注射（OV10，10mg/kg；OV8，8mg/kg）的效果。结果表明，OV10组表现出与TV10组相似的CKD病理学特征，两组的尿白蛋白/肌酐比值（p<0.05）、肾小管损伤（p<0.05）和肾纤维化程度（p<0.05）均显著高于OV8组。OV10组和TV10组在尿白蛋白/肌酐比值、肾小管损伤和肾纤维化程度方面未观察到显著差异。这些发现表明，眶后注射10mg/kg ADR产生的效果与传统尾静脉注射相当。该技术操作简单，可能会提高CKD研究中的实验效率、可重复性和动物福利。总之，本研究验证了眶后注射在建立CKD模型中的实用性，表明其有潜力规范并提高未来CKD研究方案的可靠性。
