Zhang Wei, Wang Jing, Wang Xuncheng, Duan Xuwei, Peng Junbo, Zhang Xiaoming, Xing Qikai, Zhang Kaichun, Yan Jiye
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Cherry Engineering and Technical Research Center of the State Forestry and Grassland Administration, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing Engineering Research Center for Deciduous Fruit Trees, Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100093, China.
Sci Data. 2025 Jan 22;12(1):136. doi: 10.1038/s41597-025-04462-6.
Chinese cherry belongs to the family Rosaceae, genus Prunus, and has high nutritional and economic value. 'Duiying' is a Chinese cherry variety local to Beijing, and has better performance than sweet cherry in terms of disease resistance. However, disease resistance resources of 'Duiying' have not been fully exploited partially due to the lack of a high-quality genome. In this study, we report a high-quality chromosome-scale genome assembly for Chinese cherry 'Duiying', by combining PacBio HiFi, Bionano and Hi-C sequencing data. The assembled genome has a size of 1035.19 Mb, with a scaffold N50 of 28.99 Mb, and 978.61 Mb (94.54%) assembled into 32 pseudochromosomes. A total of 547.16 Mb (52.86%) sequences were identified as repetitive sequences, and 114,451 protein-coding genes were annotated. Moreover, a total of 1635 microRNA (miRNA), 6637 transfer RNA (tRNA), 38,258 ribosomal RNA (rRNA), and 169 small nuclear RNAs (snRNA) genes were identified. The genome assembly presented here provides valuable genomic resources to enhance our understanding of genetic and molecular basis of Chinese cherry.
中国樱桃属于蔷薇科李属，具有较高的营养和经济价值。‘对樱’是北京本地的中国樱桃品种，在抗病性方面比甜樱桃表现更好。然而，由于缺乏高质量的基因组，‘对樱’的抗病性资源尚未得到充分开发。在本研究中，我们通过结合PacBio HiFi、Bionano和Hi-C测序数据，报道了中国樱桃‘对樱’的高质量染色体水平基因组组装。组装后的基因组大小为1035.19 Mb，支架N50为28.99 Mb，978.61 Mb（94.54%）组装成32条假染色体。共鉴定出547.16 Mb（52.86%）的重复序列，并注释了114,451个蛋白质编码基因。此外，还鉴定出总共1635个微小RNA（miRNA）、6637个转运RNA（tRNA）、38,258个核糖体RNA（rRNA）和169个小核RNA（snRNA）基因。本文展示的基因组组装为增进我们对中国樱桃遗传和分子基础的理解提供了有价值的基因组资源。
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