Liu Qi, Wang Xiaopeng, Yekefenhazi Dinaer, Wang Jingyu, Zhong Keer, Zhang Ying, Fu Huiyun, Zhou Zhiyong, Huang Jiangfeng, Li Wanbo, Xu Xiandong
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen, 361021, China.
Fisheries Research Institute of Jiangxi Province, Nanchang, 330039, China.
Sci Data. 2025 Jun 6;12(1):949. doi: 10.1038/s41597-025-05120-7.
Chanodichthys mongolicus, a carnivorous fish belonging to the Cyprinidae family (Erythroculter), is widely distributed in reservoirs and lakes across China. However, the lack of research on whole genome assembly has impeded advancements in genetic studies for this species. In this study, we employed PacBio sequencing and Hi-C technology to assemble high-quality genomes for both female and male Chanodichthys mongolicus at the chromosome level. The assembly results revealed a male genome size of 1.10 GB with a scaffold N50 of 43 Mb, while the female genome was 1.09 GB with a scaffold N50 of 42 Mb. Both assemblies consist of 24 chromosomes and demonstrate an average genome integrity of 98.5%, as assessed by BUSCO. We annotated the male genome using a combination of ab initio predictions, protein homology comparisons, and RNAseq data, resulting in the identification of 33,581 genes, of which 88.15% were predicted to have functional roles. These findings provide a valuable resource for future research on the genetic breeding and genome evolution of Chanodichthys mongolicus.
蒙古红鲌是一种鲤科（红鲌属）肉食性鱼类，在中国各地的水库和湖泊中广泛分布。然而，由于缺乏全基因组组装研究，阻碍了该物种遗传研究的进展。在本研究中，我们采用PacBio测序和Hi-C技术，在染色体水平上为雌性和雄性蒙古红鲌组装了高质量的基因组。组装结果显示，雄性基因组大小为1.10GB，支架N50为43Mb，而雌性基因组为1.09GB，支架N50为42Mb。通过BUSCO评估，两个组装体均由24条染色体组成，平均基因组完整性为98.5%。我们结合从头预测、蛋白质同源性比较和RNAseq数据对雄性基因组进行注释，共鉴定出33581个基因，其中88.15%的基因被预测具有功能作用。这些发现为蒙古红鲌的遗传育种和基因组进化的未来研究提供了宝贵资源。
