Zhou Ziyi, Lin Yijing, Liu Na, Zhang Yiming, Li Bing, Wang Yuanfei
Qingdao Medical College, Qingdao University, Qingdao, China.
Department of Plastic, Reconstructive and Cosmetic Surgery, Xinqiao Hospital, Army Medical University, Chongqing, China.
Front Bioeng Biotechnol. 2025 Jan 8;12:1527046. doi: 10.3389/fbioe.2024.1527046. eCollection 2024.
Artificial vascular scaffolds can mimic the structure of natural blood vessels and replace the damaged vessels by implanting them at the injury site to perform the corresponding functions. Electrospinning technology can perfectly combine biological signals and topographical cues to synergistically induce directed cell migration and growth.
In this study, poly (caprolactone) (PCL) nanofibers, PCL nanofibers uniformly coated with the extracellular matrix derived from endothelial cells (ECd), and bi-directional linear gradient ECd-coated PCL nanofibers were prepared by electrospinning and electrospray techniques to evaluate their effects on the proliferation and migration of Human umbilical vein endothelial cells (HUVECs) and rapid endothelialization.
The results showed that HUVECs could successfully adhere to the surface of these three nanofibers and maintain high viability. The migration results indicated that the bidirectional linear gradient coating could accelerate the migration of HUVECs and the endothelialization process. On this basis, three types of bionic vascular scaffolds, including PCL vascular scaffold, uniform ECd-coated PCL vascular scaffold, and bi-directional linear gradient ECd-coated PCL vascular scaffold, were further prepared. The results showed that the topology and biological signal of the bi-directional linear gradient ECd-coated PCL vascular scaffold synergistically promoted the migration of HUVECs more effectively.
This provides a new way to clinically promote the structural and functional recovery of damaged vessels and develop personalized or universal artificial vascular scaffolds, which is of great importance in cardiovascular regenerative medicine.
人工血管支架可以模仿天然血管的结构，并通过将其植入损伤部位来替代受损血管，以执行相应功能。静电纺丝技术可以完美地将生物信号和拓扑线索结合起来，协同诱导细胞定向迁移和生长。
在本研究中，通过静电纺丝和电喷雾技术制备了聚己内酯（PCL）纳米纤维、均匀涂覆有源自内皮细胞的细胞外基质（ECd）的PCL纳米纤维以及双向线性梯度涂覆ECd的PCL纳米纤维，以评估它们对人脐静脉内皮细胞（HUVECs）增殖和迁移以及快速内皮化的影响。
结果表明，HUVECs能够成功粘附在这三种纳米纤维的表面并保持高活力。迁移结果表明，双向线性梯度涂层可以加速HUVECs的迁移和内皮化过程。在此基础上，进一步制备了三种类型的仿生血管支架，包括PCL血管支架、均匀涂覆ECd的PCL血管支架和双向线性梯度涂覆ECd的PCL血管支架。结果表明，双向线性梯度涂覆ECd的PCL血管支架的拓扑结构和生物信号更有效地协同促进了HUVECs的迁移。
这为临床上促进受损血管的结构和功能恢复以及开发个性化或通用的人工血管支架提供了一种新方法，这在心血管再生医学中具有重要意义。
