Coradduzza Donatella, Cruciani Sara, Di Lorenzo Biagio, De Miglio Maria Rosaria, Zinellu Angelo, Maioli Margherita, Medici Serenella, Erre Gian Luca, Carru Ciriaco
Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy.
Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 Sassari, Italy.
Noncoding RNA. 2025 Jan 14;11(1):5. doi: 10.3390/ncrna11010005.
Rheumatoid arthritis (RA) is a chronic autoimmune disorder associated with an increased risk of cardiovascular disease (CVD), largely driven by peripheral endothelial dysfunction (ED). Humanin, a mitochondrial-derived peptide, has been suggested to play a protective role in endothelial function. However, the relationship between Humanin levels and ED in RA, as well as the interaction between Humanin and non-coding RNAs such as Long Non-Coding RNA GAS5, microRNA-21 (miR-21), and microRNA-103 (miR-103), remains unclear. This study aimed to investigate the relationship between circulating Humanin levels, non-coding RNAs (GAS5, miR-21, miR-103), and endothelial dysfunction (ED) in patients with RA. Additionally, we explored the correlation between Humanin expression and specific non-coding RNAs (GAS5, miR-21, and miR-103) to better understand their potential role in vascular health. Peripheral ED was assessed using flow-mediated pulse amplitude tonometry, with Ln-RHI values <0.51 indicating dysfunction. Humanin levels, GAS5, miR-21, and miR-103 were measured in RA patients. Univariate and multivariate analyses were conducted to determine the relationship between these biomarkers and ED. Kaplan-Meier survival analysis and ROC curve analysis were used to assess the prognostic value of Humanin. Higher Humanin levels were significantly associated with better endothelial function (OR = 0.9774, = 0.0196). Kaplan-Meier analysis demonstrated that higher Humanin levels correlated with improved survival ( < 0.0001). The non-coding RNAs (GAS5, miR-21, and miR-103) did not show significant associations with ED. Humanin is a potential protective biomarker for endothelial dysfunction and survival in RA patients. Further research is needed to explore the interaction between Humanin and non-coding RNAs in the context of vascular health.
类风湿性关节炎（RA）是一种慢性自身免疫性疾病，与心血管疾病（CVD）风险增加相关，这在很大程度上是由外周内皮功能障碍（ED）驱动的。人胰岛素（Humanin）是一种线粒体衍生肽，已被认为在内皮功能中起保护作用。然而，RA患者中Humanin水平与ED之间的关系，以及Humanin与长链非编码RNA GAS5、微小RNA-21（miR-21）和微小RNA-103（miR-103）等非编码RNA之间的相互作用仍不清楚。本研究旨在调查RA患者循环中Humanin水平、非编码RNA（GAS5、miR-21、miR-103）与内皮功能障碍（ED）之间的关系。此外，我们探讨了Humanin表达与特定非编码RNA（GAS5、miR-21和miR-103）之间的相关性，以更好地了解它们在血管健康中的潜在作用。使用血流介导的脉搏振幅张力测量法评估外周ED，Ln-RHI值<0.51表明功能障碍。在RA患者中测量了Humanin水平、GAS5、miR-21和miR-103。进行单变量和多变量分析以确定这些生物标志物与ED之间的关系。使用Kaplan-Meier生存分析和ROC曲线分析来评估Humanin的预后价值。较高的Humanin水平与较好的内皮功能显著相关（OR = 0.9774，P = 0.0196）。Kaplan-Meier分析表明，较高的Humanin水平与生存率提高相关（P < 0.0001）。非编码RNA（GAS5、miR-21和miR-103）与ED未显示出显著关联。Humanin是RA患者内皮功能障碍和生存的潜在保护生物标志物。需要进一步研究以探索在血管健康背景下Humanin与非编码RNA之间的相互作用。
