Xin Haoyang, Strickland Luke W, Hamilton John P, Trusky Jacob K, Fang Chao, Butler Nathaniel M, Douches David S, Buell C Robin, Jiang Jiming
Department of Plant Biology, Michigan State University, East Lansing, MI, USA.
Center for Applied Genetic Technologies, University of Georgia, Athens, GA, USA.
Plant Biotechnol J. 2025 Apr;23(4):1243-1256. doi: 10.1111/pbi.14582. Epub 2025 Jan 23.
Potato (Solanum tuberosum) is the third-most important food crop in the world. Although the potato genome has been fully sequenced, functional genomics research of potato lags behind that of other major food crops, largely due to the lack of a model experimental potato line. Here, we present a diploid potato line, 'Jan,' which possesses all essential characteristics for facile functional genomics studies. Jan exhibits a high level of homozygosity after seven generations of self-pollination. Jan is vigorous, highly fertile and produces tubers with outstanding traits. Additionally, it demonstrates high regeneration rates and excellent transformation efficiencies. We generated a chromosome-scale genome assembly for Jan, annotated its genes and identified syntelogs relative to the potato reference genome assembly DMv6.1 to facilitate functional genomics. To miniaturize plant architecture, we developed two 'mini-Jan' lines with compact and dwarf plant stature through CRISPR/Cas9-mediated mutagenesis targeting the Dwarf and Erecta genes involved in growth. One mini-Jan mutant, mini-Jan, is fully fertile and will permit higher-throughput studies in limited growth chamber and greenhouse space. Thus, Jan and mini-Jan offer a robust model system that can be leveraged for gene editing and functional genomics research in potato.
马铃薯（Solanum tuberosum）是世界上第三重要的粮食作物。尽管马铃薯基因组已被完全测序，但马铃薯的功能基因组学研究落后于其他主要粮食作物，这主要是由于缺乏一个模式实验马铃薯品系。在此，我们展示了一个二倍体马铃薯品系“Jan”，它具有便于进行功能基因组学研究的所有基本特征。经过七代自花授粉后，Jan表现出高度的纯合性。Jan生长旺盛、育性高，结出的块茎具有优异的性状。此外，它还表现出高再生率和出色的转化效率。我们为Jan生成了一个染色体水平的基因组组装，注释了其基因，并鉴定了相对于马铃薯参考基因组组装DMv6.1的同线基因，以促进功能基因组学研究。为了使植株结构小型化，我们通过CRISPR/Cas9介导的诱变技术，靶向参与生长的矮化基因（Dwarf）和直立基因（Erecta），培育出了两个植株紧凑且矮小的“迷你Jan”品系。其中一个迷你Jan突变体，即mini-Jan，育性完全正常，能够在有限的生长室和温室空间内进行更高通量的研究。因此，Jan和mini-Jan提供了一个强大的模式系统，可用于马铃薯的基因编辑和功能基因组学研究。
