Kaur Jaspreet, Pandove Gulab, Sidhu Amandeep Singh, Brar Sukhdeep Kaur, Singh Nitin Dev, Arora Vidhi, Walia Sohan Singh
Department of Microbiology, Punjab Agricultural University, Ludhiana, 141004, India.
School of Organic Farming, Punjab Agricultural University, Ludhiana, 141004, India.
Curr Microbiol. 2025 Jan 23;82(3):101. doi: 10.1007/s00284-025-04078-4.
Endophytes are bacteria that inhabit host plants for most of their life cycle without causing harm. In the study, 15 endophytic bacteria were isolated from 30 forage Sorghum plants and assessed for various plant growth-promoting (PGP) traits, such as phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, ammonia production, siderophore production, gibberellic acid production, Indole-3-acetic acid (IAA) production, and zinc solubilization. One isolate, JJG_Zn, demonstrated multiple PGP activities and was identified as Enterobacter sp. through 16S rRNA sequencing. Considering the concept of 'One health,' a comprehensive biosafety analysis for any plant growth-promoting bacteria (PGP bacteria) is essential, thus, Enterobacter sp. (JJG_Zn), along with two other standard cultures, Burkholderia seminalis and Stenotrophomonas maltophilia, were evaluated for biosafety tests-including sheep blood agar assay, E.coli sensitivity assay, and pathogenicity tests on albino mice. The results indicated that all three PGP bacteria were potentially innocuous and compatible with each other. Furthermore, the field experiments were conducted at Punjab Agricultural University, Regional Research Station, Bathinda, and School of Organic Farming, IFS, Punjab Agricultural University, Ludhiana during Kharif 2022 employing a randomized complete block design (RCBD) with nine treatment combinations. The treatments included liquid bacterial inoculants (LBIs) of Stenotrophomonas maltophilia, Burkholderia seminalis, and Enterobacter sp. (JJG_Zn) applied in combination with 100% and 75% of the recommended dose of fertilizer (RDF), with each treatment replicated thrice. The treatment T (75% RDF + dual inoculation with B. seminalis and S. maltophilia) resulted in the highest productivity, showing a 10.23% increase in pooled green fodder yield (GFY) compared to the control. The treatment T (75% RDF + Enterobacter sp. (JJG_Zn)) showed a 5.14% increase in productivity over the control. Thus, liquid bacterial inoculants (LBIs) present a promising approach to enhance the productivity of forage Sorghum.
内生菌是一类在其生命周期的大部分时间里寄生于宿主植物且不会造成危害的细菌。在这项研究中，从30株饲用高粱植株中分离出15种内生细菌，并对其多种促进植物生长（PGP）特性进行了评估，如解磷、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性、产氨、产铁载体、产赤霉素、产吲哚-3-乙酸（IAA）以及解锌。其中一个分离株JJG_Zn表现出多种PGP活性，并通过16S rRNA测序鉴定为肠杆菌属。考虑到“同一个健康”的理念，对任何促进植物生长的细菌（PGP细菌）进行全面的生物安全性分析至关重要，因此，对肠杆菌属（JJG_Zn）以及另外两种标准培养物——稻生伯克霍尔德菌和嗜麦芽窄食单胞菌进行了生物安全性测试，包括羊血琼脂试验、大肠杆菌敏感性试验以及对白化小鼠的致病性试验。结果表明，这三种PGP细菌都可能是无害的且彼此相容。此外，2022年雨季期间，在旁遮普农业大学巴辛达地区研究站以及旁遮普农业大学卢迪亚纳分校有机农业学院进行了田间试验，采用随机完全区组设计（RCBD），有九种处理组合。处理包括嗜麦芽窄食单胞菌、稻生伯克霍尔德菌和肠杆菌属（JJG_Zn）的液体细菌接种剂（LBI），分别与100%和75%的推荐施肥量（RDF）组合施用，每个处理重复三次。处理T（75% RDF + 稻生伯克霍尔德菌和嗜麦芽窄食单胞菌双重接种）的生产力最高，与对照相比，混合青饲料产量（GFY）提高了10.23%。处理T（75% RDF + 肠杆菌属（JJG_Zn））的生产力比对照提高了5.14%。因此，液体细菌接种剂（LBI）是提高饲用高粱生产力的一种有前景的方法。
