Sohn Min-Young, Jeong Ji-Min, Kang Gyoungsik, Woo Won-Sik, Kim Kyung-Ho, Son Ha-Jeong, Park Chan-Il
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, Republic of Korea.
Aquatic Disease Control Division, National Fishery Products Quality Management Service (NFQS), 337, Haeyang-ro, Yeongdo-gu, Busan, 49111, Republic of Korea.
Fish Shellfish Immunol. 2025 Mar;158:110149. doi: 10.1016/j.fsi.2025.110149. Epub 2025 Jan 21.
Dendritic cells (DCs) play a pivotal role in activating naïve T-cells and bridging innate and adaptive immunity. This study aimed to identify and characterize CD83 and CD276 as potential markers for DCs in olive flounder (Paralichthys olivaceus). Specific antibodies against these markers were developed and used to analyze their distribution in peripheral blood leukocytes (PBLs) and intestinal tissues. Flow cytometry and immunohistochemistry revealed that CD83 and CD276 are expressed on DCs, with peak expression observed one week after oral administration of an inactivated viral hemorrhagic septicemia virus (VHSV) vaccine. Gene expression analysis further demonstrated significant activation of immune-related genes, including CD3, IgM, and TLRs, indicating that oral vaccine administration effectively activates the intestinal mucosal immune system. These findings provide valuable insights into fish immune responses and establish CD83 and CD276 as promising DC markers, contributing to the development of mucosal vaccine strategies in aquaculture.
树突状细胞（DCs）在激活初始T细胞以及连接天然免疫和适应性免疫方面发挥着关键作用。本研究旨在鉴定和表征CD83和CD276作为牙鲆（Paralichthys olivaceus）中DCs的潜在标志物。针对这些标志物开发了特异性抗体，并用于分析它们在外周血白细胞（PBLs）和肠道组织中的分布。流式细胞术和免疫组织化学显示，CD83和CD276在DCs上表达，在口服灭活病毒性出血性败血症病毒（VHSV）疫苗一周后观察到表达峰值。基因表达分析进一步证明了包括CD3、IgM和Toll样受体（TLRs）在内的免疫相关基因的显著激活，表明口服疫苗给药有效地激活了肠道黏膜免疫系统。这些发现为鱼类免疫反应提供了有价值的见解，并确立了CD83和CD276作为有前景的DC标志物，有助于水产养殖中黏膜疫苗策略的开发。
