Chen Lingling, Wang Tao, Chen Meifang, Feng Cheng, Wang Jianzhe, Shi Zhenyu, Wu Ye
Fujian Key Laboratory of Oral Diseases & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian Province, 350002, China.
Fuzhou Hospital of Traditional Chinese Medicine Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province, 350002, China.
Clin Oral Investig. 2025 Jan 24;29(1):80. doi: 10.1007/s00784-025-06180-0.
Both the Masquelet technique (MT) and concentrated growth factors (CGF) reduce early graft loss and improve bone regeneration. This study aims to explore the efficacy of combining MT with CGF for mandibular defect repair by characterizing the induced membrane and assessing in vivo osteogenesis.
Three experimental groups were compared: negative control (NC), MT, and Masquelet combined with CGF (MTC). Four weeks after the first surgery, histopathology is used to identify the morphological structure of the induced membrane, evaluate the degree of vascularization, and the secretion levels of osteogenesis and angiogenesis-related growth factors. In vivo osteogenesis was assessed with a second autologous bone graft surgery 4 weeks later, and bone reconstruction was evaluated by micro-CT and histopathology.
CGF significantly increased the induced membrane thickness, vascularization, and growth factor secretion levels. Quantitative micro-CT analysis showed that the bone volume fraction (BV/TV) at 4 weeks post-surgery was higher in the MTC group (23.30 ± 1.15%) compared to the MT group (16.50 ± 1.29%) and NC group (12.62 ± 1.23%) (P < 0.05). By 12 weeks, the difference in BV/TV between MTC (32.59 ± 0.11%) and MT (29.89 ± 0.49%) reduced, indicating consistent bone regeneration. Trabecular parameters were consistently higher in the MTC group, highlighting enhanced osteogenesis.
Combining the Masquelet technique with CGF effectively reduces early bone graft absorption and promotes bone repair. These findings suggest potential benefits for oral and maxillofacial bone defect treatment, though further studies are needed to confirm long-term efficacy.
Integrating Masquelet technique and CGF in mandibular reconstruction may improve clinical outcomes by enhancing bone regeneration and reducing graft failure.
骨诱导膜技术（MT）和浓缩生长因子（CGF）均可减少早期移植物丢失并促进骨再生。本研究旨在通过对诱导膜进行特征分析并评估体内成骨情况，探讨MT联合CGF修复下颌骨缺损的疗效。
比较三个实验组：阴性对照组（NC）、MT组和MT联合CGF组（MTC）。首次手术后4周，采用组织病理学方法确定诱导膜的形态结构，评估血管化程度以及成骨和血管生成相关生长因子的分泌水平。4周后进行第二次自体骨移植手术评估体内成骨情况，并通过显微CT和组织病理学评估骨重建情况。
CGF显著增加了诱导膜的厚度、血管化程度和生长因子分泌水平。定量显微CT分析显示，术后4周时，MTC组的骨体积分数（BV/TV）（23.30±1.15%）高于MT组（16.50±1.29%）和NC组（12.62±1.23%）（P<0.05）。到12周时，MTC组（32.59±0.11%）和MT组（29.89±0.49%）之间的BV/TV差异减小，表明骨再生情况一致。MTC组的小梁参数始终较高，突出了其增强的成骨作用。
MT联合CGF可有效减少早期骨移植吸收并促进骨修复。这些发现表明对口腔颌面部骨缺损治疗具有潜在益处，不过需要进一步研究来证实长期疗效。
在下颌骨重建中整合骨诱导膜技术和CGF可能通过增强骨再生和减少移植失败来改善临床结果。
