Effect of exercise training intensity on adipose tissue hormone sensitive lipase gene expression in obese women under weight loss.

BACKGROUND

Hormone sensitive lipase (HSL) is an enzyme that regulates adipose tissue lipolysis and plays an important role in chronic exercise-induced changes in adipose tissue metabolism. The purpose of this study was to determine whether aerobic exercise intensity influences abdominal adipose tissue gene expression in obese women under weight loss.

METHODS

Thirty women (body mass index (BMI) = 33.0 ± 0.7 kg/m, age = 58 ± 1 years) completed one of three 20-week interventions: caloric restriction alone (CR only, = 8), CR plus moderate-intensity exercise (CR + moderate-intensity, 45%-50% heart rate reserve (HRR), 3 day/week, = 9), or CR plus vigorous-intensity exercise (CR + vigorous-intensity, 70%-75% HRR, 3 day/week, = 13). Each group had a similar prescribed energy deficit comprised of underfeeding alone (2800 kcal/week for CR only) or underfeeding (2400 kcal/week) plus exercise (400 kcal/week). Body composition and maximal aerobic capacity (VOmax) were measured, and subcutaneous abdominal adipose tissue samples were collected before and after the interventions. Adipose tissue gene expression was measured by real time reverse-transcriptase polymerase chain reaction.

RESULTS

All three interventions reduced body weight, fat mass, percent fat, and waist to a similar degree (all < 0.01). In addition, all interventions did not change absolute VOmax, but increased relative VOmax ( < 0.05 to < 0.01). Compared to pre-intervention, neither CR only nor CR + moderate-intensity changed adipose tissue gene expression, but CR + vigorous-intensity significantly increased adipose tissue gene expression ( < 0.01). The changes of gene expression levels in the CR + vigorous-intensity group were significantly different from those in the CR only ( < 0.05) and CR + moderate-intensity ( < 0.01) groups. In the whole cohort, changes in adipose tissue gene expression correlated positively to changes in absolute ( = 0.55, < 0.01) and relative ( = 0.32, = 0.09) VOmax.

CONCLUSION

These results support a potential effect of aerobic exercise training intensity on hormone sensitive lipase pathway in adipose tissue metabolism in obese women under weight loss.