Walter Charles Emmanuel Jebaraj, Shankaran Zioni Sangeetha, Kontham Sai Sushmitha, Ramachandran Kotteeswaran, Prakash Nandini, Johnson Thanka, Jr Sri Nisha
Department of Biotechnology, Sri Ramachandra Institute of Higher Education & Research (formerly Sri Ramachandra Medical College & Research Institute), Chennai, India.
Biomedical Sciences, Faculty of Allied Health Sciences, Sree Balaji Medical College & Hospital, Chennai, India.
Heliyon. 2024 Dec 27;11(1):e41519. doi: 10.1016/j.heliyon.2024.e41519. eCollection 2025 Jan 15.
The cancers of the gastrointestinal (GI) tract have become a common diagnosis worldwide contributing to a large number of mortalities. Though potentially curable they are mostly fatal due to late diagnosis and lack of accurate diagnostic markers. microRNA, micromanagers of gene expression have been associated to have distinct roles as oncogenes or tumour suppressors in several cancers including GI cancers. These miRNAs are known to harbour single nucleotide polymorphisms (SNPs) that lead to loss or gain of its functions and have been found to be associated with altering susceptibility of several cancers. The current study aimed to investigate the role of miRSNPs in common gastrointestinal cancers. A case control study was designed which included 210 GI cancer cases and 230 cancer free controls. The miRSNPs were successfully genotyped using MassARRAY technique. Association analysis revealed that 6a; rs11614913, pre-mir-423; rs6505162, pre-mir-605; rs2043556, pre-mir-149; rs2292832 and pri-mir-30c; rs928508 polymorphisms significantly altered the risk of common GI cancers. Multifactor dimensionality reduction analysis demonstrated that miRSNPs alter GI cancer risk by interacting with exposures like diabetes mellitus, alcohol consumption, diet and socioeconomic status in the study subjects. In conclusion it was found that presence of miRNA polymorphism and certain lifestyle factors alters susceptibility to GI cancers significantly.
胃肠道（GI）癌症已成为全球常见的诊断疾病，导致大量死亡。尽管有可能治愈，但由于诊断延迟和缺乏准确的诊断标志物，它们大多是致命的。微小RNA作为基因表达的微观管理者，在包括胃肠道癌症在内的几种癌症中作为癌基因或肿瘤抑制因子发挥着不同的作用。已知这些微小RNA含有单核苷酸多态性（SNP），会导致其功能丧失或获得，并且已发现与改变几种癌症的易感性有关。当前的研究旨在调查微小RNA单核苷酸多态性（miRSNP）在常见胃肠道癌症中的作用。设计了一项病例对照研究，其中包括210例胃肠道癌症病例和230例无癌对照。使用MassARRAY技术成功对miRSNP进行了基因分型。关联分析显示，6a；rs11614913、前体微小RNA-423；rs6505162、前体微小RNA-605；rs2043556、前体微小RNA-149；rs2292832和初级微小RNA-30c；rs928508多态性显著改变了常见胃肠道癌症的风险。多因素降维分析表明，miRSNP通过与研究对象中的糖尿病、饮酒、饮食和社会经济地位等暴露因素相互作用来改变胃肠道癌症风险。总之，发现微小RNA多态性的存在和某些生活方式因素会显著改变对胃肠道癌症的易感性。
