miR-605 rs2043556 与乳腺癌易感性显著相关。
Significant Association of miR-605 rs2043556 with Susceptibility to Breast Cancer.
机构信息
Genetics Division, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.
出版信息
Microrna. 2020;9(2):133-141. doi: 10.2174/2211536608666190926155149.
BACKGROUND
MicroRNAs (miRNAs) are noncoding RNA molecules, which directly regulate gene expression. It has been documented that single nucleotide polymorphisms in miRNA genes could alter the regulation of miRNA expression and function.
OBJECTIVE
In this study, the allele and genotype frequency of miR-605 rs2043556 and its association with breast cancer were investigated in the Iranian population.
METHODS
Genotyping was performed in 162 females affected with breast cancer and 180 healthy individuals. Genotyping was performed using Restriction Fragment Length Polymorphism (RFLP) followed by Sanger sequencing.
RESULTS
The data showed the presence of Hardy Weinberg equilibrium (HWE) for this marker in the Iranian population. Allelic frequency for A and G allele was 0.75 and 0.25, respectively. Odd ratios for the association between miR-605 rs2043556 AG/GG genotypes was 3.86 with p-value= 0.
CONCLUSION
The results indicated an increased risk for breast cancer susceptibility for miR-605 rs2043556 in the Iranian population.
背景
MicroRNAs (miRNAs) 是一类非编码 RNA 分子，可直接调控基因表达。已有研究证实 miRNA 基因的单核苷酸多态性可改变 miRNA 的表达和功能调控。
目的
本研究旨在探讨 miR-605 rs2043556 单核苷酸多态性在伊朗人群中的等位基因和基因型频率及其与乳腺癌的相关性。
方法
采用限制性片段长度多态性（RFLP）联合 Sanger 测序法对 162 例乳腺癌患者和 180 例健康对照者进行 miR-605 rs2043556 基因分型。
结果
该标记在伊朗人群中符合 Hardy-Weinberg 平衡（HWE）。A 和 G 等位基因的频率分别为 0.75 和 0.25。miR-605 rs2043556 的 AG/GG 基因型与乳腺癌易感性之间的比值比为 3.86，P 值为 0。
结论
miR-605 rs2043556 多态性增加了伊朗人群乳腺癌的易感性。
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