Park Sunjae, Son Youngjun, Park Jonggyu, Lee Soyoon, Kim Na-Hyeon, Jang Se-Na, Kang Tae-Woong, Song Jeong-Eun, Khang Gilson
Department of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of Korea.
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of Korea.
Gels. 2025 Jan 7;11(1):42. doi: 10.3390/gels11010042.
Articular cartilage faces challenges in self-repair due to the lack of blood vessels and limited chondrocyte concentration. Polydeoxyribonucleotide (PDRN) shows promise for promoting chondrocyte growth and cartilage regeneration, but its delivery has been limited to injections. Continuous PDRN delivery is crucial for effective cartilage regeneration. This study explores using gelatin methacrylate (gelMA) hydrogel, crosslinked with visible light and riboflavin 5'-phosphate sodium (RF) as a photoinitiator, for sustained PDRN release. GelMA hydrogel's synthesis was confirmed through spectrophotometric techniques, demonstrating successful methacrylate group incorporation. PDRN-loaded gelMA hydrogels displayed varying pore sizes, swelling ratios, degradation rates, and mechanical properties based on gelMA concentration. They showed sustained PDRN release and biocompatibility, with the 14% gelMA-PDRN composition performing best. Glycosaminoglycan (GAG) activity was higher in PDRN-loaded hydrogels, indicating a positive effect on cartilage formation. RT-PCR analysis revealed increased expression of cartilage-specific genes (COL2, SOX9, AGG) in gelMA-PDRN. Histological assessments in a rabbit cartilage defect model demonstrated superior regenerative effects of gelMA-PDRN hydrogels. This study highlights the potential of gelMA-PDRN hydrogels in cartilage tissue engineering, providing a promising approach for effective cartilage regeneration.
由于缺乏血管和软骨细胞浓度有限，关节软骨在自我修复方面面临挑战。聚脱氧核糖核苷酸（PDRN）在促进软骨细胞生长和软骨再生方面显示出前景，但其递送方式一直局限于注射。持续递送PDRN对于有效的软骨再生至关重要。本研究探索使用与可见光交联并以5'-磷酸核黄素钠（RF）作为光引发剂的甲基丙烯酸明胶（gelMA）水凝胶来实现PDRN的持续释放。通过分光光度技术确认了gelMA水凝胶的合成，证明甲基丙烯酸酯基团成功引入。基于gelMA浓度，负载PDRN的gelMA水凝胶表现出不同的孔径、溶胀率、降解速率和力学性能。它们显示出PDRN的持续释放和生物相容性，其中14%的gelMA - PDRN组合物表现最佳。负载PDRN的水凝胶中糖胺聚糖（GAG）活性较高，表明对软骨形成有积极作用。RT-PCR分析显示gelMA - PDRN中软骨特异性基因（COL2、SOX9、AGG）的表达增加。在兔软骨缺损模型中的组织学评估证明了gelMA - PDRN水凝胶具有优异的再生效果。本研究突出了gelMA - PDRN水凝胶在软骨组织工程中的潜力，为有效的软骨再生提供了一种有前景的方法。
