Carrillo González Nidia Jannette, Reyes Gutierrez Gabriela Stefania, Campos-Ordoñez Tania, Castro-Torres Rubén D, Beas Zárate Carlos, Gudiño-Cabrera Graciela
Laboratorio de Desarrollo y Regeneración Neural, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan 45220, Jalisco, Mexico.
Laboratorio de Neurobiotecnología, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan 45220, Jalisco, Mexico.
Curr Issues Mol Biol. 2025 Jan 9;47(1):35. doi: 10.3390/cimb47010035.
Olfactory ensheathing cells (OECs) and mesenchymal stem cells (MSCs) differentiated towards Schwann-like have plasticity properties. These cells express the Glial fibrillary acidic protein (GFAP), a type of cytoskeletal protein that significantly regulates many cellular functions, including those that promote cellular plasticity needed for regeneration. However, the expression of GFAP isoforms (α, β, and δ) in these cells has not been characterized. We evaluated GFAP isoforms (α, β, and δ) expression by Polymerase Chain Reaction (PCR) assay in three conditions: (1) OECs, (2) cells exposed to OECs-conditioned medium and differentiated to Schwann-like cells (dBM-MSCs), and (3) MSC cell culture from rat bone marrow undifferentiated (uBM-MSCs). First, the characterization phenotyping was verified by morphology and immunocytochemistry, using p75, CD90, and GFAP antibodies. Then, we found the expression of GFAP isoforms (α, β, and δ) in the three conditions; the expression of the GFAPα (10.95%AUC) and GFAPβ (9.17%AUC) isoforms was predominantly in OECs, followed by dBM-MSCs (α: 3.99%AUC, β: 5.66%AUC) and uBM-MSCs (α: 2.47%AUC, β: 2.97%AUC). GFAPδ isoform has a similar expression in the three groups (OEC: 9.21%AUC, dBM-MSCs: 11.10%AUC, uBM-MSCs: 9.21%AUC). These findings suggest that expression of different GFAPδ and GFAPβ isoforms may regulate cellular plasticity properties, potentially contributing to tissue remodeling processes by OECs, dBM-MSCs, and uBM-MSCs.
嗅鞘细胞（OECs）和向雪旺样细胞分化的间充质干细胞（MSCs）具有可塑性。这些细胞表达胶质纤维酸性蛋白（GFAP），这是一种细胞骨架蛋白，可显著调节许多细胞功能，包括促进再生所需的细胞可塑性的功能。然而，这些细胞中GFAP亚型（α、β和δ）的表达尚未得到表征。我们通过聚合酶链反应（PCR）检测，在三种条件下评估GFAP亚型（α、β和δ）的表达：（1）OECs，（2）暴露于OECs条件培养基并分化为雪旺样细胞的细胞（dBM-MSCs），以及（3）大鼠骨髓未分化的MSC细胞培养物（uBM-MSCs）。首先，使用p75、CD90和GFAP抗体，通过形态学和免疫细胞化学验证表征表型。然后，我们在三种条件下发现了GFAP亚型（α、β和δ）的表达；GFAPα（曲线下面积[AUC]为10.95%）和GFAPβ（AUC为9.17%）亚型的表达主要在OECs中，其次是dBM-MSCs（α：AUC为3.99%，β：AUC为5.66%）和uBM-MSCs（α：AUC为2.47%，β：AUC为2.97%）。GFAPδ亚型在三组中的表达相似（OEC：AUC为9.21%，dBM-MSCs：AUC为11.10%，uBM-MSCs：AUC为9.21%）。这些发现表明，不同的GFAPδ和GFAPβ亚型的表达可能调节细胞可塑性，可能有助于OECs、dBM-MSCs和uBM-MSCs的组织重塑过程。
