Omari Alzahrani Fatima
Department of Biology, Faculty of Sciences, Al-Baha University, Al-Baha 65729, Saudi Arabia.
Curr Issues Mol Biol. 2025 Jan 16;47(1):59. doi: 10.3390/cimb47010059.
Understanding the ammonium (NH) uptake and transport systems, particularly genes, is important for plant growth and defense. However, there is a lack of research on identifying and analyzing genes in pomegranate, emphasizing the need for further investigation in this area. Five genes ( to ) were identified, all of which contain the PF00909 domain, a feature of ammonium transporters. Various characteristics of these genes, including gene length, coding sequence length, and chromosomal locations, were examined. This study evaluated the isoelectric point, hydropathicity, conserved domains, motifs, and synteny of the PgAMT1 proteins. Phylogenetic analysis confirmed the homology of genes with previously reported in Arabidopsis and tomato. The tissue-specific expression analysis of genes revealed distinct patterns: and were predominantly expressed in flowers, exhibited notable expression in roots, leaves, and flowers, was primarily expressed in leaf tissue, while the expression of was detected in both leaves and roots. The impact of salt-induced stress on gene expression was also examined, revealing that , , and expression is reduced under increased salt stress. These expression modifications can help regulate NH+ assimilation in conditions of elevated salinity, maintaining cellular homeostasis and ion balance. This study contributes to the comprehensive identification of the s gene family in pomegranate; however, further research on the functional characterization of the identified s is needed.
了解铵（NH）吸收和转运系统，特别是相关基因，对植物生长和防御至关重要。然而，石榴中相关基因的鉴定和分析研究尚缺，这凸显了该领域进一步研究的必要性。鉴定出了五个相关基因（从 到 ），它们均含有PF00909结构域，这是铵转运蛋白的一个特征。研究了这些基因的各种特性，包括基因长度、编码序列长度和染色体定位。本研究评估了PgAMT1蛋白的等电点、亲水性、保守结构域、基序和共线性。系统发育分析证实了这些基因与拟南芥和番茄中先前报道的相关基因具有同源性。这些基因的组织特异性表达分析揭示了不同的模式： 和 主要在花中表达， 在根、叶和花中均有显著表达， 主要在叶组织中表达，而 在叶和根中均有表达。还研究了盐胁迫对相关基因表达的影响，结果表明，在盐胁迫增加时， 、 和 的表达会降低。这些表达变化有助于在盐度升高的条件下调节NH+同化，维持细胞内稳态和离子平衡。本研究有助于全面鉴定石榴中的相关基因家族；然而，还需要对已鉴定的相关基因进行功能特性的进一步研究。
