Zhou Zheng, Yong Kang, Luo Zhengzhong, Du Zhenlong, Zhou Tao, Li Xiaoping, Yao Xueping, Shen Liuhong, Yu Shumin, Huang Yixin, Cao Suizhong
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China .
Metabolites. 2025 Jan 16;15(1):58. doi: 10.3390/metabo15010058.
BACKGROUND/OBJECTIVES: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement for alleviating excessive lipid mobilisation in periparturient dairy cows. This study aimed to investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, and plasma metabolites in dairy cows.
Thirty cows were randomly divided into three groups: H-DBT group, L-DBT group, and control group. DBT administration was started on the day of calving and continued once daily for seven days. Faecal and blood samples were collected on calving day, 7 days after calving, and 14 days after calving. The levels of serum biochemical indices were measured at three time points in the three groups using commercial kits. Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing.
Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations ≥ 1 mmol/L. Additionally, DBT increased serum total cholesterol contents at both 7 and 14 days postpartum. Analysis of the microbiota community showed that DBT modulated the composition and structure of the hindgut microbiota. Metabolomic analysis revealed decreased plasma acetylcarnitine, 2-hydroxybutyric acid, and BHB levels 7 days postpartum, whereas the TCA cycle was enhanced. At 14 days postpartum, DBT altered the plasma bile acid profile, especially glycine-conjugated bile acids, including GCDCA, GUDCA, and GDCA. Correlation analyses showed that the relative abundances of , , and were strongly correlated with the differential metabolites, which is crucial for the beneficial effects of DBT.
DBT improves energy status and lipid metabolism in postpartum dairy cows by modulating hindgut microbiota and serum lipid metabolites.
背景/目的：从非泌乳状态过渡到泌乳状态是奶牛脂质代谢的关键时期。当归补血汤（DBT）可刺激能量代谢，改善与脂质代谢紊乱相关的疾病，有望成为缓解围产期奶牛过度脂质动员的有效补充剂。本研究旨在探讨补充DBT对奶牛血清生化指标、粪便微生物群落和血浆代谢物的影响。
30头奶牛随机分为三组：高剂量DBT组、低剂量DBT组和对照组。在产犊当天开始给予DBT，每天一次，持续7天。在产犊当天、产犊后7天和产犊后14天采集粪便和血液样本。使用商用试剂盒在三个时间点测量三组血清生化指标水平。选择高剂量DBT组和对照组的奶牛进行代谢组学和16S rRNA扩增子测序。
我们的研究表明，在产后7天的奶牛中，DBT显著降低了血清3-羟基丁酸（BHB）浓度以及BHB浓度≥1 mmol/L的奶牛数量。此外，DBT在产后7天和14天均增加了血清总胆固醇含量。微生物群落分析表明，DBT调节了后肠微生物群的组成和结构。代谢组学分析显示，产后7天血浆乙酰肉碱、2-羟基丁酸和BHB水平降低，而三羧酸循环增强。在产后14天，DBT改变了血浆胆汁酸谱，尤其是甘氨酸结合胆汁酸，包括甘氨鹅脱氧胆酸、甘氨熊脱氧胆酸和甘氨脱氧胆酸。相关性分析表明，[具体菌种名称未给出]、[具体菌种名称未给出]和[具体菌种名称未给出]的相对丰度与差异代谢物密切相关，这对DBT的有益作用至关重要。
DBT通过调节后肠微生物群和血清脂质代谢物来改善产后奶牛的能量状态和脂质代谢。
