Shi Yanan, Wu Chenglei, Liu Ting, Wang Rongyun, Ding Bin, Sun Qiuhua
The College of Nursing, Zhejiang Chinese Medical University, Hangzhou 310053, China.
College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Metabolites. 2025 Jan 20;15(1):65. doi: 10.3390/metabo15010065.
Postoperative ileus (POI) is a common postoperative clinical complication that significantly affects postoperative rehabilitation and quality of life in patients and can even produce secondary complications, leading to serious consequences. External treatment using Shenhuang Plaster (SHP) (Shenque acupoint administration) has definite effects and unique advantages in the prevention and treatment of POI, but its mechanism is not completely clear. In this study, we investigated the therapeutic mechanism behind the effect of Shenhuang Plaster applied to the Shenque acupoint on gastrointestinal motility in POI mice based on metabolomics.
C57BL/6 mice were divided into three groups: blank control (Ctrl), model (POI), and intervention (POI + SHP) groups. SHP treatment was started 3 days before modeling. We employed several behavioral tests and gastrointestinal transit function measurements and performed qRT-PCR analysis, 16S rRNA gene sequencing, and metabolomics analysis on serum metabolites.
We found that SHP could reduce the mRNA expression of inflammatory mediators in the smooth muscle tissue of the small intestine, regulate the structure and function of the intestinal microbiota, and modulate serum phenylalanine, carnitine, and glutamic acid levels.
POI mice had obvious intestinal flora disorders and metabolic disorders of amino acids and their derivatives, and there was a significant correlation between differential flora and differential metabolites. SHP could effectively regulate the concentration of intestinal flora and serum metabolites and the metabolic pathway related to amino acids in vivo and, ultimately, achieve a therapeutic purpose in POI. In this study, it was found, for the first time, that applying SHP to the Shenque acupoint could effectively regulate the serum metabolites of phenylalanine, carnitine, and glutamate, and improve postoperative intestinal motile disturbance through association with the intestinal flora.
术后肠梗阻（POI）是一种常见的术后临床并发症，严重影响患者术后康复及生活质量，甚至可引发继发性并发症，导致严重后果。神黄膏（SHP）（神阙穴给药）外治法在POI的防治中具有确切疗效和独特优势，但其作用机制尚不完全明确。本研究基于代谢组学探讨神阙穴贴敷神黄膏对POI小鼠胃肠动力影响的治疗机制。
将C57BL/6小鼠分为三组：空白对照（Ctrl）组、模型（POI）组和干预（POI + SHP）组。在建模前3天开始进行SHP治疗。我们采用了多种行为测试和胃肠转运功能测量，并对血清代谢物进行了qRT-PCR分析、16S rRNA基因测序和代谢组学分析。
我们发现SHP可降低小肠平滑肌组织中炎症介质的mRNA表达，调节肠道微生物群的结构和功能，并调节血清苯丙氨酸、肉碱和谷氨酸水平。
POI小鼠存在明显的肠道菌群紊乱以及氨基酸及其衍生物的代谢紊乱，差异菌群与差异代谢物之间存在显著相关性。SHP可有效调节体内肠道菌群和血清代谢物的浓度以及与氨基酸相关的代谢途径，最终实现对POI的治疗目的。本研究首次发现神阙穴贴敷SHP可有效调节血清苯丙氨酸、肉碱和谷氨酸代谢物，并通过与肠道菌群关联改善术后肠道运动障碍。
Zotero 插件
