González-Troncoso María Paz, Landeta-Salgado Catalina, Munizaga Javiera, Hornedo-Ortega Ruth, García-Parrilla María Del Carmen, Lienqueo María Elena
Department of Chemical Engineering, Biotechnology, and Materials, Centre for Biotechnology and Bioengineering (CeBiB), University of Chile, Beauchef 851, Santiago 8370456, Chile.
Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González nº 2, 41012 Sevilla, Spain.
J Fungi (Basel). 2024 Dec 24;11(1):3. doi: 10.3390/jof11010003.
Natural compounds derived from microorganisms, especially those with antioxidant and anticancer properties, are gaining attention for their potential applications in biomedical, cosmetic, and food industries. Marine fungi, such as , are particularly promising due to their ability to produce bioactive metabolites through the degradation of marine algal polysaccharides. This study investigates the metabolic diversity of grown on different carbon sources: glucose, spp., and . Crude extracts of fungal biomass were analyzed for total phenolic content (TPC), antioxidant capacity (TAC), toxicity, and phenolic compound identification using ultra-high-performance liquid chromatography coupled with high-resolution electrospray ionization mass spectrometry (UHPLC-MS/MS). The analysis revealed the presence of anthraquinone compounds, including emodin (0.36 ± 0.08 mg/g DW biomass) and citrereosein in glucose medium and citrereosein and endocrocin in medium. No such compounds were detected in spp. medium. The glucose-grown extract exhibited the highest TPC (3.09 ± 0.04 mg GAE/g DW) and TAC (39.70 ± 1.0 µmol TEq/g biomass). Additionally, no detrimental effects were observed on a neuronal cell line. These findings highlight the influence of carbon sources on the production of bioactive metabolites and their functional properties, providing valuable insights into the biotechnological potential of .
源自微生物的天然化合物，尤其是那些具有抗氧化和抗癌特性的化合物，因其在生物医学、化妆品和食品工业中的潜在应用而受到关注。海洋真菌，如 ，由于其能够通过降解海洋藻类多糖产生生物活性代谢物而特别有前景。本研究调查了 在不同碳源（葡萄糖、 属物种和 ）上生长时的代谢多样性。使用超高效液相色谱-高分辨率电喷雾电离质谱联用仪（UHPLC-MS/MS）对真菌生物质的粗提物进行总酚含量（TPC）、抗氧化能力（TAC）、毒性分析以及酚类化合物鉴定。分析揭示了蒽醌类化合物的存在，包括在葡萄糖培养基中的大黄素（0.36±0.08毫克/克干重生物质）和柑桔红素，以及在 培养基中的柑桔红素和内色菌素。在 属物种培养基中未检测到此类化合物。在葡萄糖上生长的提取物表现出最高的TPC（3.09±0.04毫克没食子酸当量/克干重）和TAC（39.70±1.0微摩尔TEq/克生物质）。此外，未观察到对神经元细胞系有有害影响。这些发现突出了碳源对生物活性代谢物产生及其功能特性的影响，为 的生物技术潜力提供了有价值的见解。
