Maranesi Margherita, Dall'Aglio Cecilia, Moscatelli Sara, Palmioli Elisa, Coliolo Paola, Marini Daniele, Guelfi Gabriella, Scocco Paola, Mercati Francesca
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, Via Pontoni 5, 62032 Camerino, Italy.
Vet Sci. 2025 Jan 10;12(1):41. doi: 10.3390/vetsci12010041.
Ghrelin (GhRL) is an orexigenic hormone influenced by nutritional state. It plays a role in skin repair and diseases, though little information exists regarding its function in this organ. GhRL and its receptor were investigated in the skin of sheep under different feeding conditions to explore GhRL system presence and possible modifications due to diet. Three-year-old female sheep were free to graze from June to the pasture maximum flowering (MxF group) and from this period to maximum dryness addicted (Exp group) or not (MxD group) with 600 gr/die/head of barley and corn. Skin samples were processed for immunohistochemistry and real-time PCR. The immunostaining showed the presence of the GhRL system in skin appendages. Indeed, the ligand was localized in the hair follicles whereas the receptor was also observed in sweat glands and smooth muscle cells. The expression of both genes was significantly higher in the Exp group (3.6 and 2.9 folds respectively, < 0.05) compared with the MxF group. These results suggest that the GhRL system is involved in the regulation of hair follicles and sweat glands. In addition, diet supplementation may positively modulate the expression of GhRL and its receptor in the skin.
胃饥饿素（GhRL）是一种受营养状态影响的促食欲激素。它在皮肤修复和疾病中发挥作用，尽管关于其在该器官中的功能信息很少。研究了不同饲养条件下绵羊皮肤中的GhRL及其受体，以探索GhRL系统的存在以及饮食引起的可能变化。三岁雌性绵羊于6月开始自由放牧至牧场最大花期（MxF组），此后根据是否添加600克/头/天的大麦和玉米进入最大干燥期（实验性添加组（Exp组）或不添加组（MxD组））。对皮肤样本进行免疫组织化学和实时PCR处理。免疫染色显示皮肤附属器中存在GhRL系统。实际上，配体定位于毛囊，而受体也在汗腺和平滑肌细胞中观察到。与MxF组相比，Exp组中两个基因的表达均显著更高（分别为3.6倍和2.9倍，<0.05）。这些结果表明，GhRL系统参与毛囊和汗腺的调节。此外，饮食补充可能会正向调节皮肤中GhRL及其受体的表达。
