Ye Zhengqin, Zhu Zhenbang, Yu Liangzheng, Zhang Zhendong, Li Xiangdong
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225012, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225012, China.
Vet Sci. 2025 Jan 13;12(1):52. doi: 10.3390/vetsci12010052.
Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry. The killed PRRSV vaccine has been reported to be safe and could elicit humoral responses. The killed PRRSV vaccine with a high viral antigen load combined with robust adjuvants could provide good protection against the infection. However, the high virus titer of PRRSV on the successive production cell lines is the prerequisite for this strategy. In this study, we explored PRRSV production in two recombinant Marc-145 cell lines expressing Nsp2 or Nsp4 through a lentivirus system. The results demonstrated that either Nsp2 or Nsp4 expressing Marc-145 cells did not affect cell morphology and growth kinetics but significantly enhanced PRRSV replication. Overall, our exploration may enable the production of high-yield PRRSV and offer a potential tool for developing safer and more effective PRRSV vaccines.
猪繁殖与呼吸综合征（PRRS）给养猪业造成了巨大的经济损失。据报道，灭活的PRRSV疫苗是安全的，并且能够引发体液免疫反应。具有高病毒抗原载量并结合强效佐剂的灭活PRRSV疫苗能够提供良好的抗感染保护。然而，在连续传代的生产细胞系上获得高病毒滴度的PRRSV是该策略的前提条件。在本研究中，我们通过慢病毒系统探索了在两种表达Nsp2或Nsp4的重组Marc-145细胞系中生产PRRSV的情况。结果表明，表达Nsp2或Nsp4的Marc-145细胞均不影响细胞形态和生长动力学，但显著增强了PRRSV的复制。总体而言，我们的探索可能有助于实现高产PRRSV的生产，并为开发更安全、更有效的PRRSV疫苗提供一个潜在工具。
