Chang BoYoon, Hwang Yuri, Kim In, Park Hyungmin, Kim Yonghwan, Kim SungYeon
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 54538, South Korea.
ForBioKorea Co., Ltd., 917, 14, Gasan digital 2-ro, Geumcheon-gu, Seoul, 08592, South Korea.
Arch Dermatol Res. 2025 Jan 24;317(1):304. doi: 10.1007/s00403-024-03656-z.
Hair follicle growth depends on the intricate interaction of cells within the follicle and its vascular supply. Current FDA-approved treatments like minoxidil have limitations, including side effects and the need for continuous use. Moracin M, a compound from Moraceae family, was investigated for its effects on hair growth and vascular regeneration. In our study, Moracin M significantly increased cell proliferation in human dermal papilla cells (hDPCs) during both the anagen and catagen phases and promoted cell migration in human umbilical vein endothelial cells (HUVECs) without cytotoxicity at concentrations up to 50 µM. Mechanistic analysis revealed that moracin M enhanced Wnt3a, GSK-3β phosphorylation and increased non-phospho β-catenin levels, activating Wnt signaling and upregulating transcription factors LEF, TCF, and AXIN2. This resulted in elevated levels of growth factors VEGF, FGF2, KGF, HGF and MYC in hDPCs, effects comparable to those of minoxidil. Additionally, moracin M significantly increased protein and mRNA levels of VEGF, FGF2, and KGF in hDPCs under IFN-γ-induced inflammatory conditions. Moracin M treatments also resulted in notable wound width reductions in a dose-dependent manner. Further investigation showed that moracin M stimulated MMP-2 and MMP-9 expression. These findings indicate that moracin M significantly enhances hair growth through the promotion of cell proliferation and angiogenesis, particularly via the activation of the Wnt signaling pathway in dermal papilla cells, presenting it as a promising therapeutic alternative to current treatments.
毛囊的生长取决于毛囊内细胞及其血管供应之间复杂的相互作用。目前美国食品药品监督管理局（FDA）批准的治疗方法，如米诺地尔，存在局限性，包括副作用以及需要持续使用。对桑科植物中的化合物桑辛素M对毛发生长和血管再生的作用进行了研究。在我们的研究中，桑辛素M在生长期和退行期均显著增加人真皮乳头细胞（hDPCs）的细胞增殖，并促进人脐静脉内皮细胞（HUVECs）的细胞迁移，在浓度高达50μM时无细胞毒性。机制分析表明，桑辛素M增强了Wnt3a、GSK - 3β磷酸化并增加了非磷酸化β - 连环蛋白水平，激活了Wnt信号并上调了转录因子LEF、TCF和AXIN2。这导致hDPCs中生长因子VEGF、FGF2、KGF、HGF和MYC水平升高，其效果与米诺地尔相当。此外，在IFN - γ诱导的炎症条件下，桑辛素M显著增加hDPCs中VEGF、FGF2和KGF的蛋白质和mRNA水平。桑辛素M治疗还以剂量依赖的方式显著减小了伤口宽度。进一步研究表明，桑辛素M刺激了MMP - 2和MMP - 9的表达。这些发现表明，桑辛素M通过促进细胞增殖和血管生成，特别是通过激活真皮乳头细胞中的Wnt信号通路，显著促进毛发生长，使其成为当前治疗方法的一种有前景的替代疗法。
