Zhang Bing, Li Zhe, Meng Chao, Zhang Guangchao, Kang Jiyu, Zhou Huacheng
Department of Pain, Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Department of Anesthesiology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
Cell Biochem Biophys. 2025 Jan 24. doi: 10.1007/s12013-025-01671-z.
This study aimed to observe the mechanism of hydrogen (H) in a lung transplantation model simulated by pulmonary microvascular endothelial cells (PMVECs), which were divided into 5 groups. The blank group was the normal PMVECs. During cold ischemia period, PMVECs in the control, O, or H groups were aerated with no gas, O, or 3% H, and 3% H after transfected with a small interfering RNA targeting Nrf2 in the H+si-Nrf2 group. Treatment with O and H decreased the oxidative stress injury, inflammation, cell apoptosis, and attenuated energy metabolism compared with the control group (P < 0.05). And the H group showed a better outcome with the increased protein expression of the Nrf2 and HO-1, which were conversed in the H+si-Nrf2 group. In conclusion, H attenuated inflammation, oxidative stress injury, cell apoptosis, and maintained the balance between energy supply and demand in a rat PMVECs lung transplantation model via Nrf2/HO-1.
本研究旨在观察氢气（H）在肺微血管内皮细胞（PMVECs）模拟的肺移植模型中的作用机制，将PMVECs分为5组。空白组为正常PMVECs。在冷缺血期，对照组、氧气组或氢气组的PMVECs分别通入无气体、氧气或3%氢气，氢气+小干扰RNA靶向Nrf2组在转染靶向Nrf2的小干扰RNA后通入3%氢气。与对照组相比，氧气和氢气处理均降低了氧化应激损伤、炎症、细胞凋亡，并减轻了能量代谢（P<0.05）。氢气组Nrf2和HO-1蛋白表达增加，结果更佳，而在氢气+小干扰RNA靶向Nrf2组中这些作用被逆转。总之，在大鼠PMVECs肺移植模型中，氢气通过Nrf2/HO-1减轻炎症、氧化应激损伤、细胞凋亡，并维持能量供需平衡。
