垂体中间部功能障碍马匹与年龄匹配对照的粪便微生物群特征及比较

Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age-matched controls.

作者信息

Wang Wenqing, Gibson Justine, Horsman Sara, Mikkelsen Deirdre, Bertin François-René

机构信息

School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.

School of Agriculture and Food Sciences, The University of Queensland, St Lucia, Queensland, Australia.

出版信息

J Vet Intern Med. 2025 Jan-Feb;39(1):e17288. doi: 10.1111/jvim.17288.

DOI:10.1111/jvim.17288

PMID:39853825

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11758151/

Abstract

BACKGROUND

Altered gut microbiota has been associated with dopaminergic degenerative diseases in people, but studies on horses with pituitary pars intermedia dysfunction (PPID) are lacking.

HYPOTHESIS/OBJECTIVES: Investigate the effect of PPID on fecal microbiota in horses.

ANIMALS

Nine horses with PPID and 13 age-matched control horses.

METHODS

Prospective control study. Fecal samples were collected bimonthly. Microbial analysis used 16S rRNA sequencing to determine the relative abundance at genus and phylum levels, assess alpha and beta diversity and identify core microbiota.

RESULTS

Horses with PPID had decreased relative abundances of Christensenellaceae R-7 group (median; 95% confidence interval [CI]: PPID, 2.04; 1.82-2.35 vs control, 2.54; 2.37-2.76; P = .02) and NK4A214 group (PPID, 2.21; 2.02-2.56 vs control, 2.62; 2.44-2.85; P = .05), and significant lower abundances of Romboutsia (log2FoldChange = -3.54; P = .04) and Peptococcaceae uncultured (log2FoldChange = -0.89; P = .04) by differential abundance analysis. However, the abundance of Fibrobacter (log2FoldChange = 0.74; P = .04) was significantly higher in the PPID group. A significant effect of PPID on beta diversity was observed (P = .004), whereas alpha diversity varied with months (P = .001). Seven unique genera were identified in horses with PPID and 12 in control horses.

CONCLUSIONS AND CLINICAL IMPORTANCE

The fecal microbial composition is altered in horses with PPID. These findings support the potential role of the microbiota-gut-brain axis in the pathogenesis of PPID.

摘要

背景

肠道微生物群的改变与人类的多巴胺能退行性疾病有关，但缺乏对患有垂体中间叶功能障碍（PPID）马匹的研究。

假设/目的：研究PPID对马粪便微生物群的影响。

动物

9匹患有PPID的马和13匹年龄匹配的对照马。

方法

前瞻性对照研究。每两个月采集一次粪便样本。微生物分析采用16S rRNA测序，以确定属和门水平的相对丰度，评估α和β多样性，并鉴定核心微生物群。

结果

患有PPID的马中，克里斯滕森菌科R-7组的相对丰度降低（中位数；95%置信区间[CI]：PPID组为2.04；1.82 - 2.35，对照组为2.54；2.37 - 2.76；P = 0.02），NK4A214组也降低（PPID组为2.21；2.02 - 2.56，对照组为2.62；2.44 - 2.85；P = 0.05），通过差异丰度分析，罗姆布茨菌属（log2倍变化=-3.54；P = 0.04）和未培养的消化球菌科（log2倍变化=-0.89；P = 0.04）的丰度显著降低。然而，纤维杆菌属的丰度（log2倍变化=0.74；P = 0.04）在PPID组中显著更高。观察到PPID对β多样性有显著影响（P = 0.004），而α多样性随月份变化（P = 0.001）。在患有PPID的马中鉴定出7个独特的属，在对照马中鉴定出12个。