鸡白细胞介素-1β前体的单克隆抗体制备及抗原表位鉴定
Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β.
作者信息
Xu Mengmeng, Huang Mengmeng, Wang Guodong, Han Jingzhe, Niu Xinxin, Han Jinze, Yu Hangbo, Zhang Yulong, Liu Runhang, Wu Ziwen, Cui Hongyu, Zhang Yanping, Wang Suyan, Liu Yongzhen, Chen Yuntong, Duan Yulu, Li Liuan, Gao Yulong, Qi Xiaole
机构信息
Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China.
Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
出版信息
Poult Sci. 2025 Feb;104(2):104807. doi: 10.1016/j.psj.2025.104807. Epub 2025 Jan 11.
Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1β, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1β monoclonal antibody (mAb). The secreted mAb 1E12 can recognize exogenous or endogenous chicken pro-IL-1β by Western blot and IFA techniques, and for the first time, a novel antigen epitope SSLSEETFY of chicken pro-IL-1β recognized by this mAb was identified. This study not only provides an important tool for the detection and research of chicken pro-IL-1β, but also has significant implications for understanding the antigenic structure and biochemical characteristics of chicken pro-IL-1β.
前白细胞介素-1β是一种重要的炎症因子，也是检测早期促炎免疫反应的生物标志物。然而，目前缺乏针对鸡炎症因子的商业化抗体，这阻碍了对鸡炎症机制的深入探究。本研究克隆并表达了鸡前白细胞介素-1β，并建立了能够稳定分泌鸡前白细胞介素-1β单克隆抗体（mAb）的杂交瘤细胞系1E12。分泌的单克隆抗体1E12可通过蛋白质免疫印迹法（Western blot）和间接免疫荧光法（IFA）识别外源性或内源性鸡前白细胞介素-1β，并且首次鉴定出该单克隆抗体识别的鸡前白细胞介素-1β的一个新抗原表位SSLSEETFY。本研究不仅为鸡前白细胞介素-1β的检测和研究提供了重要工具，也对理解鸡前白细胞介素-1β的抗原结构和生化特性具有重要意义。
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