Zhang Xiaoting, Yan Hui, Bei Lei, Xu Guige, Zhao Mingrui, Zhang Ruihua, Meng Yu, Zhu Yanli, Xie Zhijing, Jiang Shijin
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian 271017, China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, China; Shandong Key Laboratory of Animal Microecological Preparations, Taian 271000, China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian 271017, China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Taian 271017, China.
Poult Sci. 2025 Feb;104(2):104837. doi: 10.1016/j.psj.2025.104837. Epub 2025 Jan 20.
Duck viral hepatitis (DVH) is one of the most common diseases of waterfowl. Duck hepatitis A virus type 1 (DHAV-1) and type 3 (DHAV-3) have been on the rise seriously endangering the development of duck farming. In this study, we constructed a recombinant Lactococcus lactis (L. lactis) co-expressing the fusion VP1 protein of DHAV-1 and DHAV-3. Western blot and immunofluorescence analyses confirmed that the fusion VP1 protein was expressed on the cell surface of recombinant L. lactis stain. After immunization with the recombinant L. lactis by oral administration, the ducklings were challenged with highly pathogenic DHAV-1 and DHAV-3 by natural infection. The results showed that the recombinant L. lactis induced the specific IgG antibodies, and significantly increased the level of mucosal secretory immunoglobulin A (sIgA) and cytokines, such as interleukin-4 (IL-4), interleukin-10 (IL-10), and interferon gamma (IFN-γ). The immunized ducklings delayed death for around 4 days, and reduced mortality with the relative percent survival (RPS) being 64.71 %, 61.54 % and 57.89 % in DHAV-1, DHAV-3 and DHAV-1 + 3 infection group respectively. These results showed that the recombinant L. lactis constructed in this study provides a promising candidate for prevention and control of DHAV-1 and DHAV-3 infection.
鸭病毒性肝炎（DVH）是水禽最常见的疾病之一。1型鸭甲型肝炎病毒（DHAV-1）和3型鸭甲型肝炎病毒（DHAV-3）呈上升趋势，严重危及养鸭业的发展。在本研究中，我们构建了一种共表达DHAV-1和DHAV-3融合VP1蛋白的重组乳酸乳球菌（L. lactis）。蛋白质免疫印迹和免疫荧光分析证实，融合VP1蛋白在重组乳酸乳球菌菌株的细胞表面表达。通过口服重组乳酸乳球菌对雏鸭进行免疫后，使其通过自然感染受到高致病性DHAV-1和DHAV-3的攻击。结果表明，重组乳酸乳球菌诱导产生特异性IgG抗体，并显著提高黏膜分泌型免疫球蛋白A（sIgA）和细胞因子如白细胞介素-4（IL-4）、白细胞介素-10（IL-10）和干扰素γ（IFN-γ）的水平。免疫后的雏鸭死亡延迟约4天，死亡率降低，在DHAV-1、DHAV-3和DHAV-1 + 3感染组中相对存活率（RPS）分别为64.71%、61.54%和57.89%。这些结果表明，本研究构建的重组乳酸乳球菌为预防和控制DHAV-1和DHAV-3感染提供了一个有前景的候选方案。
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