Wang Yanfei, Wang Qingwei, Ji Quan, An Pengcheng, Wang Xiaodong, Ju Yonghong, Li Ruiyang, Ruan Yong, Zhao Jiafu, Cao Maosheng, Chen Xiang
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, 550025, China; Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang, 550025, China; College of Animal Science, Guizhou University, Guiyang, 550025, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, 550025, China; Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang, 550025, China; College of Animal Science, Guizhou University, Guiyang, 550025, China.
Theriogenology. 2025 Mar 15;235:221-230. doi: 10.1016/j.theriogenology.2025.01.016. Epub 2025 Jan 17.
Oocyte quality can affect mammal fertilization rate, early embryonic development, pregnancy maintenance, and fetal development. Oxidative stress induced by reactive oxygen species (ROS) is one of the most important causes of poor oocyte maturation in vitro. To reduce the degree of cellular damage caused by ROS, supplementation with the antioxidant N-Acetyl-L-cysteine (NAC) serves as an effective pathway to alleviate glutathione (GSH) depletion during oxidative stress. This study investigated the effects of NAC supplementation during in vitro maturation of goat oocytes and explored the molecular mechanisms of maturation by transcriptome sequencing of MⅡ oocytes. The results showed that 1.5 mM NAC significantly increased the rates of oocyte maturation and cumulus cell expansion and improved the subsequent development of embryos. During the subsequent culture of parthenogenetically activated embryos, 1.5 mM NAC significantly increased the division rate of oocytes and blastocyst rate. It also reduced the accumulation of ROS, increased the level of GSH, alleviated oxidative stress, and enhanced the antioxidant capacity and cell metabolic activity. Transcriptome sequencing results revealed that NAC treatment significantly increased the expression of SIRT1, GGCT, and MITF genes related to the cellular antioxidant system, as well as the IDH3G gene related to energy metabolism, and decreased the expression of CASP8, FOS, and MMP1 genes related to apoptosis and cell invasion, as well as the CCL2. and CXCL8 genes related to the inflammatory response. In conclusion, the findings suggest that NAC supplementation significantly reduces oxidative stress, improves antioxidant capacity and metabolic activity, promotes oocyte maturation, and improves oocyte quality.
卵母细胞质量会影响哺乳动物的受精率、早期胚胎发育、妊娠维持及胎儿发育。活性氧（ROS）诱导的氧化应激是体外卵母细胞成熟不良的最重要原因之一。为降低ROS造成的细胞损伤程度，补充抗氧化剂N-乙酰-L-半胱氨酸（NAC）是减轻氧化应激期间谷胱甘肽（GSH）消耗的有效途径。本研究调查了在山羊卵母细胞体外成熟过程中补充NAC的效果，并通过对MⅡ期卵母细胞进行转录组测序探索了成熟的分子机制。结果表明，1.5 mM NAC显著提高了卵母细胞成熟率和卵丘细胞扩展率，并改善了后续胚胎发育。在孤雌激活胚胎的后续培养中，1.5 mM NAC显著提高了卵母细胞的分裂率和囊胚率。它还减少了ROS的积累，提高了GSH水平，减轻了氧化应激，并增强了抗氧化能力和细胞代谢活性。转录组测序结果显示，NAC处理显著增加了与细胞抗氧化系统相关的SIRT1、GGCT和MITF基因以及与能量代谢相关的IDH3G基因的表达，并降低了与细胞凋亡和细胞侵袭相关的CASP8、FOS和MMP1基因以及与炎症反应相关的CCL2和CXCL8基因的表达。总之，研究结果表明补充NAC可显著降低氧化应激，提高抗氧化能力和代谢活性，促进卵母细胞成熟，并改善卵母细胞质量。
