Hentges Cristiano Roberto, Schuck Laura Kauer, De Abreu Caberlon Carolina, Tozetti Alexandro Marques, Oliveira Guendalina Turcato
Conservation Physiology Laboratory, Morphological Sciences Department, Postgraduate Program in Ecology and Evolution of Biodiversity, School of Health Sciences and Life, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Universidade do Vale do Rio dos Sinos, Centro de Ciências da Saúde,PPG Biologia, São Leopoldo, RS, Brazil.
Environ Toxicol Pharmacol. 2025 Mar;114:104643. doi: 10.1016/j.etap.2025.104643. Epub 2025 Jan 22.
This study evaluated the effects of a glyphosate-based herbicide on the oxidative balance, energy metabolism, and body condition indices in tadpoles of Boana faber. Anuran spawns were collected, and after hatching and reaching Gosner stage 25, they were acclimated and exposed (168 hours) to concentrations of glyphosate (G1: 65, G2: 260, and G3: 520 µg/L). The body condition markers revealed a significant decrease in all these biomarkers in G2 and G3. Tadpoles exposed to the highest concentration of glyphosate showed an increase in superoxide dismutase activity and the maintenance of lipoperoxidation levels. Carbonyl proteins of animals of G1 and G2 groups showed a reduction that coincides with increased catalase activity. Glycogen decreased in all exposed groups, indicating that this polysaccharide was used for energy production. The body condition markers established here are a non-invasive and promising way of assessing the health status of animals.
本研究评估了一种草甘膦基除草剂对费氏雨蛙蝌蚪氧化平衡、能量代谢和身体状况指标的影响。收集蛙卵，孵化后并发育至戈斯纳25期，将蝌蚪进行驯化并暴露于不同浓度的草甘膦中（168小时）（G1：65、G2：260和G3：520μg/L）。身体状况指标显示，G2和G3组中所有这些生物标志物均显著下降。暴露于最高浓度草甘膦的蝌蚪超氧化物歧化酶活性增加，脂质过氧化水平维持不变。G1和G2组动物的羰基蛋白减少，这与过氧化氢酶活性增加相一致。所有暴露组的糖原均减少，表明这种多糖被用于能量产生。此处建立的身体状况指标是评估动物健康状况的一种非侵入性且有前景的方法。
