Ali Manar T, Al-Mahdy Dalia A, El Fishawy Ahlam M, Salama Abeer, Al-Karmalawy Ahmed A, Otify Asmaa M
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy, Cairo, 11562, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy, Cairo, 11562, Egypt; Department of Pharmacognosy, Faculty of Pharmacy, Modern University for Technology and Information (MTI University), Cairo, Egypt.
J Ethnopharmacol. 2025 Feb 27;342:119394. doi: 10.1016/j.jep.2025.119394. Epub 2025 Jan 23.
Sphagneticola trilobata was traditionally used to alleviate wounds using topical plant preparations. The precise mechanism of the plant responsible for its wound healing effect are still unclear. Although the plant was reported to be cytotoxic, there is a lack of reported data regarding its cytotoxic impact on skin cancer.
To in-depth investigate wound healing and cytotoxic effects of the plant extracts and its fractions by using different assays.
In vitro scratch assay and in vivo full-thickness wound model were performed to evaluate the plant's wound healing activity. Enzyme-linked immunosorbent assay kits were employed to assess key skin parameters levels. The cytotoxicity was evaluated using in vitro Western Standard Time assay against skin carcinoma cells.
Sphagneticola trilobata improved the wound closure percentage in both in vitro scratch assay and in vivo model by modulating inflammation, as evidenced by a reduction in tumor necrosis factor and mitogen activated protein kinase levels, and enhancing proliferation via elevating collagen, smooth muscle actin, and forkhead protein. The total extract's efficacy surpassed the effectiveness of reference ointment. The non-polar fraction outperformed the polar one in accelerating wound closure and improving the tested skin parameters levels. Trilobolide-6-O-isobutyrate displayed the best docking scores for the selected target receptors. Moreover, the non-polar fraction demonstrated the most powerful cytotoxic effect against skin cancer cells. A specific compound mixture (kaurenoic acid/grandiflorenic acid, 1 and 2) within this fraction exhibited stronger cytotoxicity.
Sphagneticola trilobata can be considered as a promising therapeutic agent in relieving wounds and combating skin cancer cell lines.
传统上，人们使用三裂叶蟛蜞菊的局部植物制剂来缓解伤口。该植物促进伤口愈合的确切机制尚不清楚。尽管据报道该植物具有细胞毒性，但缺乏关于其对皮肤癌细胞毒性影响的报道数据。
通过不同的检测方法深入研究该植物提取物及其馏分的伤口愈合和细胞毒性作用。
进行体外划痕试验和体内全层伤口模型，以评估该植物的伤口愈合活性。使用酶联免疫吸附测定试剂盒评估关键皮肤参数水平。使用体外Western标准时间测定法评估对皮肤癌细胞的细胞毒性。
三裂叶蟛蜞菊通过调节炎症，提高了体外划痕试验和体内模型中的伤口闭合率，肿瘤坏死因子和丝裂原活化蛋白激酶水平降低证明了这一点，并通过提高胶原蛋白、平滑肌肌动蛋白和叉头蛋白来促进增殖。总提取物的疗效超过了参比软膏。在加速伤口闭合和改善测试皮肤参数水平方面，非极性馏分优于极性馏分。三叶苷-6-O-异丁酸酯对选定的靶受体显示出最佳对接分数。此外，非极性馏分对皮肤癌细胞表现出最强的细胞毒性作用。该馏分中的一种特定化合物混合物（贝壳杉烯酸/大花沼兰烯酸，1和2）表现出更强的细胞毒性。
三裂叶蟛蜞菊可被视为一种有前途的治疗剂，用于缓解伤口和对抗皮肤癌细胞系。
