通过转录组鉴定出的BDH1对奶山羊乳腺上皮细胞的脂质代谢具有负面影响。

BDH1 identified by transcriptome has a negative effect on lipid metabolism in mammary epithelial cells of dairy goats.

作者信息

Ni Mengke, Zang Saige, Wang Yihan, Qin Xiaochen, Tian Min, Xiong Tiantian, Chen Chong, Zhang Yue, Luo Jun, Li Cong

机构信息

College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.

出版信息

BMC Genomics. 2025 Jan 24;26(1):66. doi: 10.1186/s12864-025-11245-1.

DOI:10.1186/s12864-025-11245-1

PMID:39856554

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11761236/

Abstract

BACKGROUND

The 3-hydroxybutyrate dehydrogenase 1 (BDH1) mainly participates in the regulation of milk fat synthesis and ketone body synthesis in mammary epithelial cells. In our previous study, BDH1 was identified as a key candidate gene regulating lipid metabolism in mammary glands of dairy goats by RNA-seq. This study aimed to investigate the effect of BDH1 on lipid metabolism in mammary epithelial cells of dairy goats (GMECs).

RESULTS

The results suggest that BDH1 plays a significant role in reducing triacylglycerol content and lipid droplet accumulation in GMECs (p < 0.05). Overexpression of BDH1 significantly decreased the expression of lipid metabolism-related genes (SREBF1 and GPAM) and reduced the levels of C14:0 and C17:1, while increasing FABP3 expression and C10:0 concentration (p < 0.05). Interference with BDH1 significantly increased the expression of SREBF1 and GPAM and the concentration of C14:0, C15:1, and C20:1, but significantly decreased FABP3 and C18:0 (p < 0.05). Treatment of GMECs with β-hydroxybutyric acid (R-BHBA) significantly decreased the expression of FASN, ACACA, LPL, SREBF1, FABP3, ACSL1, GPAM, DGAT1, and triacylglycerol content, while significantly increasing the expression of BDH1 (p < 0.05). Interference with BDH1 rescued the reduction of cellular TAG content and the expression of FASN, LPL, SREBF1, ACSL1, and GPAM in BHBA-treated GMECs.

CONCLUSION

In conclusion, BDH1 negatively regulates lipid metabolism in mammary glands of dairy goats. Furthermore, it may mitigate the inhibitory effect of R-BHBA on lipid metabolism in GMECs.

摘要

背景

3-羟基丁酸脱氢酶1（BDH1）主要参与乳腺上皮细胞中乳脂肪合成和酮体合成的调控。在我们之前的研究中，通过RNA测序将BDH1鉴定为调控奶山羊乳腺脂质代谢的关键候选基因。本研究旨在探讨BDH1对奶山羊乳腺上皮细胞（GMECs）脂质代谢的影响。

结果

结果表明，BDH1在降低GMECs中三酰甘油含量和脂滴积累方面发挥着重要作用（p < 0.05）。BDH1的过表达显著降低了脂质代谢相关基因（SREBF1和GPAM）的表达，降低了C14:0和C17:1的水平，同时增加了FABP3的表达和C10:0的浓度（p < 0.05）。干扰BDH1显著增加了SREBF1和GPAM的表达以及C14:0、C15:1和C20:1的浓度，但显著降低了FABP3和C18:0（p < 0.05）。用β-羟基丁酸（R-BHBA）处理GMECs显著降低了FASN、ACACA、LPL、SREBF1、FABP3、ACSL1、GPAM、DGAT1的表达和三酰甘油含量，同时显著增加了BDH1的表达（p < 0.05）。干扰BDH1挽救了BHBA处理的GMECs中细胞TAG含量以及FASN、LPL、SREBF1、ACSL1和GPAM表达的降低。