Pasternak L, Ristau E
Arch Geschwulstforsch. 1985;55(1):17-21.
One single subcutaneous injection of 100 micrograms protein of a glycoprotein fraction from UV light-induced mouse sarcoma produces transplantation resistance against the same tumor. The glycoprotein fraction was isolated by 2% Triton X-100 and 3M KCl from enriched membranes. As measured by immunogenicity a tenfold enrichment of a tumor-associated antigen was obtained when compared with the 3M KCl extract on the basis of protein concentration.
单次皮下注射100微克由紫外线诱导的小鼠肉瘤糖蛋白组分中的蛋白质,可产生针对同一肿瘤的移植抗性。该糖蛋白组分通过2% Triton X-100和3M KCl从富集膜中分离得到。以免疫原性衡量,与基于蛋白质浓度的3M KCl提取物相比,肿瘤相关抗原得到了10倍的富集。