γδ T细胞受慢性淋巴细胞白血病负担的影响显著，但仅受髓系来源的抑制性细胞轻度影响。

γδ T Are Significantly Impacted by CLL Burden but Only Mildly Influenced by M-MDSCs.

作者信息

Zarobkiewicz Michał, Kowalska Wioleta, Szymańska Agata, Lehman Natalia, Kowalczyk Bożena, Tomczak Waldemar, Bojarska-Junak Agnieszka

机构信息

Department of Clinical Immunology, Medical University of Lublin, 20-093 Lublin, Poland.

Department of Genetics and Microbiology, Maria Curie-Sklodowska University, 20-033 Lublin, Poland.

出版信息

Cancers (Basel). 2025 Jan 14;17(2):254. doi: 10.3390/cancers17020254.

DOI:10.3390/cancers17020254

PMID:39858035

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11763719/

Abstract

BACKGROUND/OBJECTIVES: The current study explores the impact of CLL on γδ T cells and, in an attempt to better understand the sources of immunosuppression, assesses the impact of M-MDSCs on γδ T cells in vitro.

METHODS

The study included 163 CLL patients and 34 healthy volunteers. γδ T cells were screened with flow cytometry, including NKG2D, Fas, FasL, and TRAIL staining. Additionally, to deepen understanding of the immunosuppressive impact of CLL on γδ T, a set of in vitro co-cultures of γδ T and M-MDSCs was performed.

RESULTS

RNAseq revealed significant, though relatively minor, changes in the transcriptome. Functional analyses showed a minor drop in cytotoxic potential against CLL cells. Finally, depletion of M-MDSCs from CLL-derived peripheral blood mononuclear cells did not restore γδ T cells' proliferative response.

CONCLUSIONS

Altogether, this suggests a minor impact of M-MDSCs on activated γδ T. Thus, it seems probable that other mechanisms than M-MDSCs mediate the negative impact of CLL on circulating γδ T cells.

摘要

背景/目的：本研究探讨慢性淋巴细胞白血病（CLL）对γδT细胞的影响，并为更好地理解免疫抑制的来源，评估单核细胞来源的髓系抑制细胞（M-MDSCs）在体外对γδT细胞的影响。

方法

本研究纳入了163例CLL患者和34名健康志愿者。采用流式细胞术对γδT细胞进行筛选，包括自然杀伤细胞2D（NKG2D）、Fas、Fas配体（FasL）和肿瘤坏死因子相关凋亡诱导配体（TRAIL）染色。此外，为了更深入地了解CLL对γδT细胞的免疫抑制作用，进行了一组γδT细胞与M-MDSCs的体外共培养实验。