Qi Haole, Wang Sining, Liu Yuehan, Wang Xueping, Li Xiaoxia, Shi Fengling
Key Laboratory of Grassland Resources of the Ministry of Education, College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010010, China.
Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Grassland Research Center, National Forestry and Grassland Administration, Beijing 100091, China.
Int J Mol Sci. 2025 Jan 20;26(2):838. doi: 10.3390/ijms26020838.
is one of the typical ecological grass species, characterized by its strong salt tolerance. Hexokinase (HXK) plays a crucial role in plant growth, development, and resistance to abiotic stresses. To understand the function of in the salt tolerance of , this study identified and analyzed the gene family members using the whole-genome data of . Additionally, transcriptomic methods were employed to investigate the expression levels and stress response patterns of the family genes under salt stress. The results showed that 25 genes were identified in the gene family, which were classified into three subfamilies based on the phylogenetic tree. Members within the same subfamily exhibited similar gene structures and conserved motifs. The promoter regions of contained numerous cis-regulatory elements associated with plant hormone responses, plant growth and development, and resistance to abiotic stresses. Quantitative real-time PCR analysis provided preliminary evidence that the , , and genes might play important roles in the salt tolerance regulation of . These findings offer a theoretical foundation for further elucidating the functions and molecular regulatory mechanisms of under salt stress. This study has provided a theoretical basis for the breeding of new varieties of ecological restoration grasses with stronger salt tolerance and better growth and development. This is of great significance for the improvement and ecological restoration of saline-alkali land.
是典型的生态草种之一，具有很强的耐盐性。己糖激酶（HXK）在植物生长、发育及抗非生物胁迫中起关键作用。为了解[草种名称]在耐盐性中的功能，本研究利用[草种名称]的全基因组数据鉴定并分析了[HXK]基因家族成员。此外，采用转录组学方法研究了盐胁迫下[HXK]家族基因的表达水平和应激反应模式。结果表明，在[HXK]基因家族中鉴定出25个[HXK]基因，根据系统发育树将其分为三个亚家族。同一亚家族内的成员表现出相似的基因结构和保守基序。[HXK]的启动子区域含有许多与植物激素反应、植物生长发育及抗非生物胁迫相关的顺式调控元件。定量实时PCR分析提供了初步证据，表明[具体基因名称1]、[具体基因名称2]和[具体基因名称3]基因可能在[草种名称]的耐盐性调控中发挥重要作用。这些发现为进一步阐明[草种名称]在盐胁迫下的功能和分子调控机制提供了理论基础。本研究为培育耐盐性更强、生长发育更好的生态修复草新品种提供了理论依据。这对盐碱地的改良和生态修复具有重要意义。
