Bulama Modu Mustapha, Yiğit Hanoğlu Duygu, Hanoğlu Azmi, Alkaş Fehmi Burak, Başer K Hüsnü Can, Özkum Yavuz Dudu
Department of Pharmaceutical Botany, Faculty of Pharmacy, Near East University, 99010 Nicosia, Cyprus.
Department of Pharmacognosy, Faculty of Pharmacy, Near East University, 99010 Nicosia, Cyprus.
Molecules. 2025 Jan 15;30(2):332. doi: 10.3390/molecules30020332.
The genus L'Hér., is native to Australia with 61 introduced taxa in Cyprus, including Luehm., which has a wide distribution on the island. The aim of this study was to investigate the possible seasonal variations in the chemical composition of the essential oils of juvenile and mature leaves collected from Nicosia, Cyprus, by using multivariate statistical analysis. The leaves of 12 monthly collections were separately hydrodistilled, and GC-FID and GC/MS analyses were conducted. In general, the results revealed 1,8-cineole (mature: 3.6-27.8%; juvenile: 12.7-21.5%) and torquatone (mature: 27.6-48.8%; juvenile: 28.8-41.5%) as major compounds as well as an inverse relation between 1,8-cineole and torquatone content. Other important compounds found were α-pinene, β-eudesmol and α-eudesmol for all samples. The data support the existence of three major clusters, distinguished by the concentration of torquatone and miniatone. Minor compounds were also temporally relevant. The present study is among the first of its kind, analyzing the essential oils for a one-year period in Cyprus as well as conducting statistical analysis on to reveal possible temporal variations between heterophyllous leaves, and also performing Hierarchical Cluster Analysis, determining the primary components of variability.
L'Hér.属原产于澳大利亚，在塞浦路斯有61个引入的分类群，包括在该岛广泛分布的Luehm.属。本研究的目的是通过多元统计分析，调查从塞浦路斯尼科西亚采集的幼叶和成熟叶精油化学成分可能存在的季节性变化。对12个月采集的叶片分别进行水蒸馏，并进行气相色谱-火焰离子化检测（GC-FID）和气相色谱-质谱联用（GC/MS）分析。总体而言，结果显示1,8-桉叶素（成熟叶：3.6 - 27.8%；幼叶：12.7 - 21.5%）和扭扣酮（成熟叶：27.6 - 48.8%；幼叶：28.8 - 41.5%）为主要成分，且1,8-桉叶素和扭扣酮含量呈负相关。所有样品中还发现了其他重要成分，如α-蒎烯、β-桉叶醇和α-桉叶醇。数据支持存在三个主要聚类，以扭扣酮和小钮酮的浓度区分。次要成分在时间上也具有相关性。本研究是同类研究中的首批研究之一，对塞浦路斯一年期间的精油进行分析，并对揭示异形叶之间可能的时间变化进行统计分析，还进行层次聚类分析，确定变异的主要成分。
