Damulienė Vaida, Kaškonienė Vilma, Kaškonas Paulius, Mickienė Rūta, Maruška Audrius
Instrumental Analysis Open Access Centre, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
Institute of Metrology, Kaunas University of Technology, LT-51368 Kaunas, Lithuania.
Pharmaceuticals (Basel). 2024 Dec 26;18(1):15. doi: 10.3390/ph18010015.
A variety of phytochemicals from different plants are collected by bees into bee pollen granules. This research focused on evaluating the effects of lactic acid fermentation and enzymatic hydrolysis on the antibacterial activity of bee pollen and its interaction with antibiotics. There is limited knowledge regarding the interactions between treated bee pollen extracts and antibiotics, and this study contributes to the field by providing new insights into the antibacterial activity of pollen subjected to eight distinct treatment methods. : Bee pollen's bacterial fermentation using a culture and spontaneous fermentation were performed. Bee pollen hydrolysis was performed using commercial enzymes, including enzyme mixtures as well as pure enzymes. The agar well diffusion assay was employed to assess the antibacterial activity against , , and serovars Enteritidis and Typhimurium, as well as their interaction with antibiotics (ceftazidime, ciprofloxacin, oxytetracycline dihydrate, and erythromycin). : This study showed an enhancement in bee pollen's antibacterial activity after both fermentation and enzymatic hydrolysis. The increase varied with the pollen's origin, treatment type, and culture used for antimicrobial tests. More than 77% of bee pollen extracts demonstrated a synergistic effect with antibiotics across all tested bacterial strains, while antagonistic interactions were comparatively rare. : The applied treatment methods can improve the antibacterial properties of bee pollen. Bee pollen extracts, in combination with antibiotics, can enhance their effectiveness. These findings provide new insights into the potential use of bee pollen in combating bacterial infections.
蜜蜂从不同植物中采集多种植物化学物质并将其融入蜂花粉颗粒中。本研究聚焦于评估乳酸发酵和酶解对蜂花粉抗菌活性的影响及其与抗生素的相互作用。关于经处理的蜂花粉提取物与抗生素之间的相互作用，目前了解有限，而本研究通过对经过八种不同处理方法的花粉抗菌活性提供新见解，为该领域做出了贡献。：采用培养物对蜂花粉进行细菌发酵以及自然发酵。使用商业酶（包括酶混合物和纯酶）对蜂花粉进行水解。采用琼脂扩散法评估对肠炎沙门氏菌和鼠伤寒沙门氏菌血清型的抗菌活性，以及它们与抗生素（头孢他啶、环丙沙星、二水合土霉素和红霉素）的相互作用。：本研究表明，发酵和酶解后蜂花粉的抗菌活性均有所增强。这种增强因花粉来源、处理类型和用于抗菌测试的培养物而异。超过77%的蜂花粉提取物在所有测试细菌菌株中均与抗生素表现出协同作用，而拮抗相互作用相对较少。：所应用的处理方法可改善蜂花粉的抗菌性能。蜂花粉提取物与抗生素联合使用可增强其有效性。这些发现为蜂花粉在对抗细菌感染方面的潜在用途提供了新见解。
