Ganser A, Elstner E, Hoelzer D
Br J Haematol. 1985 Apr;59(4):627-33. doi: 10.1111/j.1365-2141.1985.tb07357.x.
The cellular composition of mixed haemopoietic colonies CFU-GEMM from the peripheral blood of normal individuals was investigated using both an improved in vitro culture assay and a monoclonal antibody reactive with the earliest megakaryocytic precursor cells. The frequency of CFU-GEMM was found to be 22 +/- 7/ml blood (mean +/- SE; n = 8) and 4.9 +/- 1.8/10(6) mononuclear cells. Double-labelling of single colonies with monoclonal antibodies against megakaryocytic as well as granulocytic cells revealed that only 18% of mixed colonies from the peripheral blood contained megakaryocytic cells as compared to 87% of mixed colonies from the bone marrow, while no difference existed with regard to granulocytic cells. It is concluded that circulating multilineage progenitor cells either require different culture conditions to express their megakaryocytic component or, alternatively, that the circulating pluripotent stem cell pool mainly consists of bipotent rather than multipotent progenitor cells.
采用改良的体外培养试验和与最早的巨核细胞前体细胞反应的单克隆抗体，对正常个体外周血中混合造血集落CFU-GEMM的细胞组成进行了研究。发现CFU-GEMM的频率为22±7/毫升血液（平均值±标准误；n = 8）和4.9±1.8/10⁶个单核细胞。用抗巨核细胞和粒细胞的单克隆抗体对单个集落进行双重标记，结果显示，外周血混合集落中只有18%含有巨核细胞，而骨髓混合集落中这一比例为87%，而粒细胞方面则没有差异。得出的结论是，循环中的多谱系祖细胞要么需要不同的培养条件来表达其巨核细胞成分，要么循环中的多能干细胞池主要由双能而非多能祖细胞组成。
