Blaj Diana-Andreea, Peptu Catalina A, Balan-Porcarasu Mihaela, Peptu Cristian, Tuchilus Cristina Gabriela, Ochiuz Lacramioara
"Petru Poni" Institute of Macromolecular Chemistry, 700487 Iasi, Romania.
Faculty of Chemical Engineering and Protection of the Environment, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania.
Pharmaceutics. 2025 Jan 17;17(1):130. doi: 10.3390/pharmaceutics17010130.
Magnolol (MG) and honokiol (HK) are bioactive compounds extracted from and trees with significant pharmacological properties, including antioxidant and antibacterial activity. However, their poor water solubility and low bioavailability limit the therapeutic potential. To address these limitations, this study aims to develop MG and HK formulations by co-electrospinning using custom-synthesized β-cyclodextrin-oligolactide (β-CDLA) derivatives. MALDI MS and NMR were employed for the structural assessment of the β-CDLA derivatives. This polymer-free electrospinning technique utilizes the high solubility of β-CDLA to incorporate MG and HK into fibrous webs. The morphology of the resulting fibers is established by SEM and further characterized using FTIR and NMR spectroscopy to confirm the successful incorporation of MG and HK. The antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, while the antimicrobial activity was evaluated against several standard microorganisms (, , , and ). The MG and HK electrospun formulations were prepared using highly concentrated feed solutions in dimethylformamide (180% w/v). The resulting β-CDLA fibers, with diameters above 400 nm and an active compound content of 7% wt., exhibited enhanced long-term antioxidant activity and improved antimicrobial efficacy, including notable activity against . This study demonstrates the potential of MG and HK-loaded β-CDLA fibrous formulations as delivery systems with prolonged antioxidant activity and notable antibacterial efficacy, providing a promising platform for biomedical applications.
厚朴酚（MG）和和厚朴酚（HK）是从厚朴树和凹叶厚朴树中提取的生物活性化合物，具有显著的药理特性，包括抗氧化和抗菌活性。然而，它们较差的水溶性和低生物利用度限制了其治疗潜力。为了解决这些限制，本研究旨在通过使用定制合成的β-环糊精-低聚丙交酯（β-CDLA）衍生物进行共电纺丝来开发MG和HK制剂。采用基质辅助激光解吸电离质谱（MALDI MS）和核磁共振（NMR）对β-CDLA衍生物进行结构评估。这种无聚合物的电纺丝技术利用β-CDLA的高溶解性将MG和HK纳入纤维网中。通过扫描电子显微镜（SEM）确定所得纤维的形态，并进一步使用傅里叶变换红外光谱（FTIR）和核磁共振光谱进行表征，以确认MG和HK的成功掺入。使用2,2-二苯基-1-苦基肼基（DPPH）自由基清除试验测定抗氧化活性，同时针对几种标准微生物（金黄色葡萄球菌、大肠杆菌、白色念珠菌和黑曲霉）评估抗菌活性。MG和HK电纺制剂使用二甲基甲酰胺中的高浓度进料溶液（180% w/v）制备。所得的β-CDLA纤维直径超过400 nm，活性化合物含量为7% wt.，表现出增强的长期抗氧化活性和改善的抗菌功效，包括对金黄色葡萄球菌的显著活性。本研究证明了负载MG和HK的β-CDLA纤维制剂作为具有延长抗氧化活性和显著抗菌功效的递送系统的潜力，为生物医学应用提供了一个有前景的平台。
