Leshkasheli Lika, Kusradze Ia, Bolkvadze Darejan, Askilashvili Lia, Chichashvili Maria, Tsertsvadze Giorgi, Zaldastanishvili Elisabed
Laboratory of Molecular Biology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, Georgia.
Laboratory of General Microbiology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, Georgia.
Viruses. 2025 Jan 15;17(1):115. doi: 10.3390/v17010115.
The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including . -targeting phages are among the most promising alternative therapy options. They have already been successfully applied in a number of cases, and it is expected that the need for anti- phages will only increase in the future. This prospect highlights the need for well-characterized therapeutic phages. In this work, we describe a phage, which also infects strains of . Here, we characterize phage M198 in terms of its biological and genetic properties. Since in some phage therapy cases, phages are administered in combination with antibiotics, here, we also screen for possible synergistic effects of combining phage M198 with six different antibiotics. We found that phage M198 has good lytic activity against clinical isolates; it does not have any indications of a temperate lifestyle, and it has synergistic potential when combined with some therapeutically relevant antibiotics.
抗生素耐药性在全球范围内的迅速传播正迅速成为人类医疗保健领域一个日益令人担忧的问题。对于许多革兰氏阴性细菌病原体，包括……靶向噬菌体是最有前途的替代治疗选择之一。它们已经在许多案例中成功应用，预计未来对抗噬菌体的需求只会增加。这一前景凸显了对特征明确的治疗性噬菌体的需求。在这项工作中，我们描述了一种噬菌体，它也感染……菌株。在这里，我们从生物学和遗传学特性方面对噬菌体M198进行了表征。由于在一些噬菌体治疗案例中，噬菌体会与抗生素联合使用，在这里，我们还筛选了噬菌体M198与六种不同抗生素联合使用时可能产生的协同效应。我们发现噬菌体M198对临床分离株具有良好的裂解活性；它没有任何温和生活方式的迹象，并且与一些具有治疗相关性的抗生素联合使用时具有协同潜力。
