Bai Shimeng, Cui Yanxin, Liao Qibin, Yi Hongyang, Liao Zhonghui, Zhang Gengwei, Wu Fenfang, Lu Hongzhou
School of Public Health, Bengbu Medical University, Bengbu 233030, China.
Bio-Therapeutic Center, Shenzhen Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China.
Viruses. 2025 Jan 16;17(1):116. doi: 10.3390/v17010116.
The re-emergence of the mpox pandemic poses considerable challenges to human health and societal development. There is an urgent need for effective prevention and treatment strategies against the mpox virus (MPXV). In this study, we focused on the A35R protein and created a chimeric A35R-Fc protein by fusing the Fc region of IgG to its C-terminal. We then assessed its reactivity with A35R-specific antibodies and human convalescent plasma, as well as its immunogenicity. Our findings indicate that the A35R-Fc protein significantly enhances affinity to A35R antibodies compared to the commercially available A35R protein and exhibits considerable reactivity to human plasma. Additionally, mice immunized with A35R-Fc exhibited increased neutralizing antibody titers against the live MPXV. These results support the potential of Fc domain chimeric antigens as a strategy to enhance the efficacy of subunit vaccines targeting the MPXV.
猴痘大流行的再度出现对人类健康和社会发展构成了巨大挑战。迫切需要针对猴痘病毒（MPXV）的有效预防和治疗策略。在本研究中，我们聚焦于A35R蛋白，并通过将IgG的Fc区域融合至其C末端，创建了一种嵌合A35R-Fc蛋白。然后，我们评估了其与A35R特异性抗体和人类康复期血浆的反应性，以及其免疫原性。我们的研究结果表明，与市售A35R蛋白相比，A35R-Fc蛋白显著增强了对A35R抗体的亲和力，并对人类血浆表现出相当的反应性。此外，用A35R-Fc免疫的小鼠对活MPXV的中和抗体滴度有所增加。这些结果支持了Fc结构域嵌合抗原作为一种增强针对MPXV的亚单位疫苗效力的策略的潜力。
