通过成像流式细胞术评估和可视化全血中细胞微聚集体形成的方案。
Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry.
作者信息
Krell Johannes, Javarappa Komal Kumar, Wenedy Angie, Frelinger Andrew L, Renia Laurent, Prazeres da Costa Clarissa, Schlegel Martin, Knolle Percy, Schneider Gerhard, Hayden Oliver
机构信息
Department of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany.
TUM CREATE Ltd, CREATE Way 10-02, CREATE Tower, Singapore.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103598. doi: 10.1016/j.xpro.2025.103598. Epub 2025 Jan 24.
Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.
血细胞聚集体在许多医学病症中是具有临床应用价值的生物标志物。本方案使用少量全血和成像流式细胞术对细胞聚集体进行准确的定量分析。我们描述了样本采集、染色和测量的步骤。然后详细介绍了门控程序和细胞形态分析。通过省略红细胞裂解和白细胞分离，同时保持白细胞和血小板的高通量精确成像，可减轻样本制备伪像、细胞激活和细胞形态变化的影响。
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