Dhanasekaran Solairaj, Liang Lisha, Chen Yaqi, Chen Jingwei, Guo Shuaiying, Zhang Xiaoyun, Zhao Lina, Zhang Hongyin
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China.
Plant Physiol Biochem. 2025 Mar;220:109531. doi: 10.1016/j.plaphy.2025.109531. Epub 2025 Jan 21.
The research intended to explore the control ability of alginate oligosaccharide (AOS) on Penicillium expansum infection in pear fruit by priming response and its mechanism. The results showed that 100 mg L AOS treatment could significantly reduce the incidence of postharvest blue mold and the lesion diameter in pear fruits and maintain their quality. The defense responses induced by AOS treatment alone were relatively mild in pear fruits. Still, AOS-treated pear fruits inoculated with P. expansum showed more intense disease resistance responses. These defense responses included enhanced activities of chitinase (CHI), β-1, 3-glucanase (GLU), peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia-lyase activity (PAL), along with the accumulation of total phenolic compounds, flavonoids and lignin. Additionally, the expression levels of defense-related genes, such as PbGLU, PbCHI, PbPAL, PbPOD and PbPPO, were significantly upregulated. However, AOS did not show a potential inhibitory effect on the in vitro growth of P. expansum. Our results indicated that AOS treatment in the postharvest pear fruit enhances disease resistance by priming its defense responses.
该研究旨在通过引发反应探讨海藻酸寡糖（AOS）对梨果实扩展青霉感染的控制能力及其机制。结果表明，100 mg/L AOS处理可显著降低采后梨果实青霉病的发病率和病斑直径，并保持其品质。单独用AOS处理诱导的防御反应在梨果实中相对较弱。然而，接种扩展青霉的AOS处理梨果实表现出更强的抗病反应。这些防御反应包括几丁质酶（CHI）、β-1,3-葡聚糖酶（GLU）、过氧化物酶（POD）、多酚氧化酶（PPO）、苯丙氨酸解氨酶活性（PAL）的增强，以及总酚类化合物、类黄酮和木质素的积累。此外，防御相关基因如PbGLU、PbCHI、PbPAL、PbPOD和PbPPO的表达水平显著上调。然而，AOS对扩展青霉的体外生长没有显示出潜在的抑制作用。我们的结果表明，采后梨果实中AOS处理通过引发其防御反应来增强抗病性。
