Tsuji K, Sowa M, Nakano R
Biol Reprod. 1985 Mar;32(2):413-7. doi: 10.1095/biolreprod32.2.413.
The relationship between the follicular size in the human ovary and oocytes capable of resuming meiosis in vitro was examined in each phase of the menstrual cycle. Intact healthy oocytes with corona cells obtained from small (3-4 mm diameter), medium (5-8 mm diameter) and large (9-15 mm diameter) antral follicles were cultured at 37 degrees C for 43 h. At the end of the culture period the denuded oocytes were examined morphologically to determine whether the resumption of meiosis had occurred. In the follicular phase, the percentage of oocytes resuming meiosis (polar body extruded: PB) in the large-follicle group was significantly higher than that in the small-follicle group (P less than 0.05). The incidence of oocyte maturation including only nuclear maturity seemed to increase as follicles increase in size. However, in the luteal phase the incidence was fairly constant, irrespective of the follicular size. These results suggest that the capacity of human oocyte maturation is closely correlated with follicular maturation.
在月经周期的每个阶段，研究了人类卵巢中卵泡大小与能够在体外恢复减数分裂的卵母细胞之间的关系。从直径小（3 - 4毫米）、中（5 - 8毫米）和大（9 - 15毫米）的窦卵泡中获取带有卵丘细胞的完整健康卵母细胞，在37℃培养43小时。培养期结束时，对去除卵丘细胞的卵母细胞进行形态学检查，以确定是否发生了减数分裂的恢复。在卵泡期，大卵泡组中恢复减数分裂（排出极体：PB）的卵母细胞百分比显著高于小卵泡组（P < 0.05）。仅包括核成熟的卵母细胞成熟发生率似乎随着卵泡大小的增加而增加。然而，在黄体期，无论卵泡大小，发生率相当恒定。这些结果表明，人类卵母细胞成熟能力与卵泡成熟密切相关。
