Xiao Dandan, Liu Jiahao, Wang Jing, Yang Xiaoqian, Yang Yuzhang, Yu Ruen, Wang Chun, Gao Hongbo, Wang Yanwei, Liu Yanping, Fan Dingchen, Lin Furong
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Genomics. 2025 Mar;117(2):111004. doi: 10.1016/j.ygeno.2025.111004. Epub 2025 Jan 23.
Gleditsia sinensis Lam. (G. sinensis) as an important species within the Leguminosae family, has been utilized in Chinese medicine for centuries, and its thorns serve as a chief medicinal ingredient. The absence of a comprehensive genome database has hindered its in-depth research. In this investigation, a chromosome-level de novo genome assembly of G. sinensis 'Yulin No.1' was achieved, which harbors a 786.13 Mb sized genome with 36,408 protein-coding genes and experiences two WGD events. The comparative and evolutionary analysis unveiled the close phylogenetic relationship between G. sinensis and eight other Leguminosae species. The WGCNA and gene family analysis further indicated that GsinMYB was involved in the development of thorns. This investigation offered a high-level genome of G. sinensis, facilitating comparisons in Leguminosae species evolution and functional elucidation. It also provided key insights for further research on the molecular regulation mechanisms of thorn development in plants and the molecular breeding of G. sinensis.
皂荚（Gleditsia sinensis Lam.）作为豆科的一个重要物种，已在中药中使用了几个世纪，其刺是主要药用成分。缺乏全面的基因组数据库阻碍了对其深入研究。在本研究中，完成了皂荚‘玉林1号’的染色体水平从头基因组组装，其基因组大小为786.13 Mb，有36408个蛋白质编码基因，经历了两次全基因组复制事件。比较和进化分析揭示了皂荚与其他八个豆科物种之间密切的系统发育关系。加权基因共表达网络分析（WGCNA）和基因家族分析进一步表明，GsinMYB参与了刺的发育。本研究提供了高质量的皂荚基因组，有助于豆科物种进化比较和功能阐释。它还为进一步研究植物刺发育的分子调控机制和皂荚分子育种提供了关键见解。
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