Petkov M, Popov G
Vet Med Nauki. 1979;16(10):74-80.
The hemagglutination activity and morphology of antigen K 99 in enterotoxic E. coli strains isolated from calves was studied. It was proven that antigen K 99 is produced not at 18 degrees C, but at 37 degrees C. All K 99+ E. coli strains react with absorbed anti K 99 serum, showing various anti-agglutination titers. The hemagglutination activity of K 99+ E. coli strains is inhibited by absorbed K 99 antiserum. In immunodiffuse tests all K 99+ E. coli strains react by producing a single precipitation line. It is proven electronmicroscopically that on the surface of the bacterial cell of K 99+ E. coli strains is observed a filamentous structure, consisting of numerous fine fibers which give it a fibriform outlook. No such morphological structures are observed in K 99- E. coli strains.
对从犊牛分离出的产肠毒素大肠杆菌菌株中抗原K99的血凝活性和形态进行了研究。已证实抗原K99不在18℃产生,而是在37℃产生。所有K99+大肠杆菌菌株都与吸收后的抗K99血清发生反应,呈现出不同的抗凝集效价。K99+大肠杆菌菌株的血凝活性被吸收后的K99抗血清所抑制。在免疫扩散试验中,所有K99+大肠杆菌菌株通过产生单一沉淀线发生反应。通过电子显微镜证实,在K99+大肠杆菌菌株的细菌细胞表面观察到一种丝状结构,它由许多细纤维组成,使其呈现出纤维状外观。在K99-大肠杆菌菌株中未观察到这种形态结构。