González-Nicolás María Ángeles, Lázaro Alberto
Renal Physiopathology Laboratory, Department of Nephrology, Instituto Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Department of Physiology, School of Medicine, Universidad Complutense, Madrid, Spain.
Renal Physiopathology Laboratory, Department of Nephrology, Instituto Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Department of Physiology, School of Medicine, Universidad Complutense, Madrid, Spain.
Methods Cell Biol. 2025;192:69-82. doi: 10.1016/bs.mcb.2024.05.004. Epub 2024 Jun 12.
Sepsis is a systemic inflammatory response to infection, and its occurrence is associated with a poor prognosis in the context of multiorgan dysfunction syndrome (MODS). Although there are several animal models for the study of its etiology, the cecal ligation and puncture (CLP) model has been considered the "Gold standard" because it shows a high degree of similarity to the progression of human sepsis. Currently, it is one of the most frequently chosen options to search for therapeutic alternatives to diminish the progression and organ damage induced by sepsis. Here, we describe in depth the CLP technique in a rat model and its application in the study of acute renal failure (ARF), the most severe complication during sepsis.
脓毒症是对感染的全身性炎症反应，在多器官功能障碍综合征（MODS）的背景下，其发生与预后不良相关。尽管有多种用于研究其病因的动物模型，但盲肠结扎穿刺（CLP）模型被认为是“金标准”，因为它与人类脓毒症的进展高度相似。目前，它是寻找治疗替代方案以减少脓毒症引起的进展和器官损伤最常选用的方法之一。在此，我们深入描述大鼠模型中的CLP技术及其在急性肾衰竭（ARF）研究中的应用，急性肾衰竭是脓毒症期间最严重的并发症。
