Krait2：一款用于微卫星研究、可视化及标记开发的多功能软件。

Krait2: a versatile software for microsatellite investigation, visualization and marker development.

作者信息

Du Lianming, Chen Jiahao, Sun Dalin, Zhao Kelei, Zeng Qianglin, Yang Nan

机构信息

Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, School of Pharmacy, Chengdu University, Chengdu, 610106, China.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu, 610225, China.

出版信息

BMC Genomics. 2025 Jan 25;26(1):72. doi: 10.1186/s12864-025-11252-2.

DOI:10.1186/s12864-025-11252-2

PMID:39863857

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11762079/

Abstract

BACKGROUND

Microsatellites are highly polymorphic repeat sequences ubiquitously interspersed throughout almost all genomes which are widely used as powerful molecular markers in diverse fields. Microsatellite expansions play pivotal roles in gene expression regulation and are implicated in various neurological diseases and cancers. Although much effort has been devoted to developing efficient tools for microsatellite identification, there is still a lack of a powerful tool for large-scale microsatellite analysis.

RESULTS

We present Krait2, a user-friendly graphical tool for investigating perfect, imperfect and compound microsatellites from FASTA and FASTQ formatted genomic datasets. Krait2 not only provides features such as primer design, repeat filtering, repeat annotation and statistical analysis, but also offers various output formats to support customized downstream analysis. Moreover, it has capability of analyzing multiple genomes simultaneously and conducting comparative analysis.

CONCLUSIONS

Krait2 is a versatile and easy-to-use software for both novices and experts to identify and analyze microsatellites. The installer and source code are available at https://github.com/lmdu/krait2 .

摘要