Evans R E, Hara T J
Brain Res. 1985 Mar 18;330(1):65-75. doi: 10.1016/0006-8993(85)90008-3.
Electro-olfactograms (EOGs) were recorded from both sensory and non-sensory epithelia on the olfactory lamellae and from other areas within the rosette of rainbow trout (Salmo gairdneri). The trout EOG induced by amino acids was a monophasic negative voltage composed of a phasic component which declined to a steady level (tonic component) that was maintained throughout stimulus duration. The time period (2 min) for complete recovery of the EOG was obtained by applying two identical stimuli (10(-5) mol/liter L-serine for 5 s) successively at increasing intervals. EOG response to amino acids increased nearly exponentially with concentration and no saturation was reached. Gradual deterioration of olfactory receptor cells occurred within 14 days after olfactory nerve section (axotomy) as indicated by phospholipid staining. Regeneration of the receptor cells started at 56 days postaxotomy, reached approximately normal density and the elongate form of mature neurons at 84 days. In axotomized fish the magnitude of EOG response decreased relative to the controls at 7 days with minimal sensitivity between 14 and 28 days postaxotomy. EOGs were not evoked by L-serine nor L-leucine in most fish 17-22 days postaxotomy. Restoration of the EOG response coincided with morphological repopulation of receptor neurons after 84 days. EOG recovery was only 50% of the control values at 230 days. The EOG responses evoked by HCl in sensory and non-sensory epithelia were indistinguishable from each other, indicating that receptor neurons are not likely to be their primary origin.
从虹鳟鱼（Salmo gairdneri）嗅叶上的感觉上皮和非感觉上皮以及嗅束内的其他区域记录了电嗅图（EOG）。氨基酸诱导的虹鳟鱼EOG是一种单相负电压，由一个相位成分组成，该成分下降到一个在整个刺激持续时间内维持的稳定水平（紧张成分）。通过以递增的间隔连续施加两个相同的刺激（10^(-5)摩尔/升L-丝氨酸，持续5秒），获得EOG完全恢复所需的时间（2分钟）。EOG对氨基酸的反应随浓度几乎呈指数增加，且未达到饱和。如磷脂染色所示，嗅神经切断（轴突切断）后14天内嗅感受器细胞逐渐退化。感受器细胞的再生在轴突切断后56天开始，在84天时达到近似正常的密度和成熟神经元的细长形态。在轴突切断的鱼中，EOG反应的幅度在7天时相对于对照组下降，在轴突切断后14至28天之间敏感性最低。在轴突切断后17 - 22天，大多数鱼对L-丝氨酸和L-亮氨酸均无EOG反应。84天后EOG反应的恢复与感受器神经元的形态再填充同时发生。在230天时，EOG恢复仅为对照值的50%。感觉上皮和非感觉上皮中HCl诱发的EOG反应彼此无法区分，这表明感受器神经元不太可能是其主要起源。
