Bagust J, Forsythe I D, Kerkut G A
Brain Res. 1985 Apr 8;331(2):315-25. doi: 10.1016/0006-8993(85)91557-4.
A detailed description is given of an hemisected spinal cord preparation from adult golden hamsters and this preparation has been used to investigate the physiology of the dorsal root reflex. In addition to antidromic reflex discharges which could be recorded from lumbar dorsal roots following stimulation of adjacent dorsal roots or the dorsal columns, spontaneous firing was also recorded from the dorsal roots. This activity reached a peak at 27 degrees C and was abolished at temperatures above 35 degrees C. Both the evoked and the spontaneous dorsal root activity were demonstrated to be travelling antidromically along the dorsal roots out of the cord, and replacement of the calcium in the bathing medium by manganese showed them to be of synaptic origin. Stimulation of a lumbar dorsal root was found to evoke a reflex in up to 4 adjacent spinal segments in both rostral and caudal directions, and a period of depressed activity was demonstrated following both evoked and spontaneous discharges. A time-locked relationship was found between the dorsal root reflex and the slow dorsal horn potential recorded from within the spinal cord.
本文详细描述了成年金黄仓鼠的半切脊髓制备方法，该制备方法已用于研究背根反射的生理学。除了在刺激相邻背根或背柱后可从腰段背根记录到的逆向反射放电外，还从背根记录到了自发放电。这种活动在27℃时达到峰值，在高于35℃的温度下消失。诱发的和自发的背根活动均被证明是沿背根逆向传出脊髓的，用锰替代浴液中的钙表明它们起源于突触。发现刺激腰段背根可在头端和尾端方向的多达4个相邻脊髓节段诱发反射，并且在诱发放电和自发放电后均出现一段活动抑制期。在背根反射与从脊髓内记录到的慢背角电位之间发现了锁时关系。
