Zhao Chunyan, Lei Shuwen, Zhao Hong, Li Zelin, Miao Yue, Peng Chunxiu, Gong Jiashun
College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Agro-products Processing Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650221, China.
Food Funct. 2025 Feb 17;16(4):1310-1329. doi: 10.1039/d4fo05947f.
The intestinal microbiota undergoes diurnal compositional and functional oscillations within a day, which affect the metabolic homeostasis of the host and exacerbate the occurrence of obesity. TB has the effect of reducing body weight and lipid accumulation, but the mechanism of improving obesity caused by a high-fat diet based on the circadian rhythm of intestinal microorganisms has not been clarified. In this study, we used multi-omics and imaging approaches to investigate the mechanism of TB in alleviating obesity in mice based on the circadian rhythm of gut microbiota. The results showed that TB could significantly regulate the levels and rhythmic expression of serum lipid indicators (TG, TC, LDL) and serum hormones (MT, FT3, LEP, CORT). The number of intestinal microbiota colonizing the colonic epithelium underwent daily fluctuations. TB remodeled the rhythmic oscillation of gut microbes (, , , .), including the number, composition, abundance and rhythmic expression of the biogeographic localization of microbes. TB notably reduced the levels of 16 bile acids (TCA, THDCA, TCDA, GHDCA, T-α-MCA, .) and restored the balance of bile acid metabolism. It was found that TB may mitigate high-fat diet-induced obesity in mice by reshaping the circadian rhythm of the gut microbiome and regulating bile acid metabolism.
肠道微生物群在一天内会经历昼夜组成和功能振荡，这会影响宿主的代谢稳态并加剧肥胖的发生。TB具有减轻体重和脂质积累的作用，但基于肠道微生物的昼夜节律改善高脂饮食引起的肥胖的机制尚未阐明。在本研究中，我们使用多组学和成像方法，基于肠道微生物群的昼夜节律来研究TB减轻小鼠肥胖的机制。结果表明，TB可显著调节血清脂质指标（TG、TC、LDL）和血清激素（MT、FT3、LEP、CORT）的水平和节律性表达。定殖于结肠上皮的肠道微生物数量呈现每日波动。TB重塑了肠道微生物的节律振荡（，，，.），包括微生物生物地理定位的数量、组成、丰度和节律性表达。TB显著降低了16种胆汁酸（TCA、THDCA、TCDA、GHDCA、T-α-MCA、.）的水平，并恢复了胆汁酸代谢的平衡。研究发现，TB可能通过重塑肠道微生物群的昼夜节律和调节胆汁酸代谢来减轻小鼠高脂饮食诱导的肥胖。
