来自贻贝壳的羟基磷灰石/β-磷酸三钙三维支架：合成、表征及细胞毒性
3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity.
作者信息
Fonseca Sabrina Cunha da, Freitas Rosangela Borges, Sotiles Anne Raquel, Schemczssen-Graeff Zelinda, Miranda Inaiê Maiala De Almeida, Biscaia Stellee Marcela Petris, Wypych Fernando, Silva Trindade Edvaldo da, Leão Moira Pedroso, Zielak João César, Franco Célia Regina Cavichiolo
机构信息
Department of Cell Biology, Federal University of Paraná, Post box-19031, Zip code -81531-970, Curitiba, PR, Brazil.
Department of Chemistry, Federal University of Paraná, Post box-19032, Zip code-81531-980, Curitiba, PR, Brazil.
出版信息
Heliyon. 2024 Dec 31;11(1):e41585. doi: 10.1016/j.heliyon.2024.e41585. eCollection 2025 Jan 15.
UNLABELLED
Bone tissue substitutes are increasing in importance. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) act as a cell matrix and improve its mechanical properties. One of their raw materials is marine-origin by-products.
OBJECTIVES
To synthesize, characterize, and evaluate the cellular cytotoxicity of a 3D biomaterial based on HA and β-TCP from mussel shells.
METHODS
We prepared pellets with 150, 200, and 250 mg and evaluated them through sintering, XRD, FTIR, ICP-OES, Scanning Electron Microscopic (SEM), and immunocytochemical tests. The Alamar Blue method was applied to the Balb-T3T cell line within 72 h to evaluate cytotoxicity.
RESULTS
Our biomaterials presented a smooth surface with slight irregularity and porosities presenting different diameters and morphologies and showed chemical, morphological, and ultrastructural similarity to bone hydroxyapatite, mainly the 150 and 200 mg pellets.
SIGNIFICANCE
We produced promising HA/β-TCP bioceramics with characteristics that allowed cell culture, promoting adhesion, spreading, and proliferation.
未标注
骨组织替代物的重要性日益增加。羟基磷灰石（HA）和β-磷酸三钙（β-TCP）可作为细胞基质并改善其力学性能。它们的原材料之一是海洋来源的副产品。
目的
合成、表征并评估一种基于贻贝壳的HA和β-TCP的3D生物材料的细胞毒性。
方法
我们制备了150、200和250毫克的颗粒，并通过烧结、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、电感耦合等离子体发射光谱（ICP-OES）、扫描电子显微镜（SEM）和免疫细胞化学测试对其进行评估。在72小时内将阿拉玛蓝法应用于Balb-T3T细胞系以评估细胞毒性。
结果
我们的生物材料表面光滑，略有不规则，孔隙呈现不同的直径和形态，并且在化学、形态和超微结构上与骨羟基磷灰石相似，主要是150毫克和200毫克的颗粒。
意义
我们制备出了具有良好前景的HA/β-TCP生物陶瓷，其特性允许细胞培养，促进细胞黏附、铺展和增殖。
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